Relation between CD43 distribution, cellular morphology, and cellular locomotion
| Secondary Antibodies Used for CD43 Labeling . | CD43 Distribution . | Cellular Morphology . | Locomotion . |
|---|---|---|---|
| Bivalent secondary antibodies “CD43 cross-linking” | Cap 94% ± 4 | Polarized 83% ± 14(a)† | 78% ± 13(a) (caps located at the uropods) |
| Round 17% ± 14(a)† | 0%(a) | ||
| Patch 6% ± 4* | Polarized 50% ± 50(b) | 17% ± 29(b) | |
| Round 50% ± 50(b) | 0%(b) | ||
| Monovalent secondary antibodies | Cap 25% ± 332-160 | Polarized 97% ± 5(a)‡ | 93% ± 9(a) (caps located at the uropods) |
| Round 2% ± 4(a)‡ | 0%(a) | ||
| Patch + Uniform 75% ± 332-160 | Polarized 15% ± 25(b)2-153 | 0%(b) | |
| Round 75% ± 25(b) | 0%(b) |
| Secondary Antibodies Used for CD43 Labeling . | CD43 Distribution . | Cellular Morphology . | Locomotion . |
|---|---|---|---|
| Bivalent secondary antibodies “CD43 cross-linking” | Cap 94% ± 4 | Polarized 83% ± 14(a)† | 78% ± 13(a) (caps located at the uropods) |
| Round 17% ± 14(a)† | 0%(a) | ||
| Patch 6% ± 4* | Polarized 50% ± 50(b) | 17% ± 29(b) | |
| Round 50% ± 50(b) | 0%(b) | ||
| Monovalent secondary antibodies | Cap 25% ± 332-160 | Polarized 97% ± 5(a)‡ | 93% ± 9(a) (caps located at the uropods) |
| Round 2% ± 4(a)‡ | 0%(a) | ||
| Patch + Uniform 75% ± 332-160 | Polarized 15% ± 25(b)2-153 | 0%(b) | |
| Round 75% ± 25(b) | 0%(b) |
During the locomotion experiments represented in Table 1, CD43 distribution was observed to be either uniform, patched, or capped. Neutrophil morphology was divided into 2 groups: cells with front-tail polarity and nonpolarized cells. Cells were locomoting or stationary. We recorded the different shape and locomotory activity associated with different patterns of CD43 distribution. The results are expressed in percent ± SD of the total cell population, or are expressed in percent ± SD of cells among the capped (a) or patched (b) cell population.
P < .001.
P < .05.
P < .005.
P < .001.
P < .025.