Effect of various antibody treatments on neutrophil locomotion
| Cellular Labeling . | None . | MEM 59 + Fab Antimouse . | MEM 59 + F(ab′)2 Antimouse . |
|---|---|---|---|
| Cell motility (% ± SD) | 22 ± 2.6a | 22 ± 28b | 73 ± 15c |
| Speed of locomoting cells (μm/min ± SD) | 10.9 ± 0.6 | 9.2 ± 1.4 | 14 ± 5.4 |
| Cellular Labeling . | None . | MEM 59 + Fab Antimouse . | MEM 59 + F(ab′)2 Antimouse . |
|---|---|---|---|
| Cell motility (% ± SD) | 22 ± 2.6a | 22 ± 28b | 73 ± 15c |
| Speed of locomoting cells (μm/min ± SD) | 10.9 ± 0.6 | 9.2 ± 1.4 | 14 ± 5.4 |
Locomotion assay of nonlabeled cells, cells labeled with anti-CD43 mAb and secondary Fab or F(ab′)2 fragments-TRITC conjugated. Cellular labeling was performed at 4°C, cells were then incubated 15 minutes at 37°C and mounted between a coverslip and a slide (serum coated). Video analyses were performed at 37°C for sequences of 10 min. The proportion of migrating cells and their speed were determined by morphometry. Table 1 represents the results of 3 replicate experiments. The differences between c and the controls (a, b) are statistically significant (P < .025). The differences between the speed of locomoting cells are not statistically significant.