Cell numbers and populations of lymphocytes in the spleen of mice recipients of bone marrow transplantation (BMT) 14 and 28 days after BMT
| Mouse group . | Day . | No. of cells (×107) . | Cell population (% of total spleen cells) . | |||||
|---|---|---|---|---|---|---|---|---|
| CD3 . | CD19 . | CD4 . | CD134+CD4 . | CD8 . | H-2Kb+d+/H-2Kb+d− . | |||
| BDF1 (control) | 7.5 ± 1.6 | 52.1 ± 7.4 | 36.4 ± 8.1 | 35.6 ± 7.2 | 3.2 ± 0.8 | 17.8 ± 4.2 | 100/0 | |
| B6 TCD-BM | 14 28 | 6.5 ± 1.2 6.8 ± 1.4 | 14.9 ± 2.2 15.2 ± 3.5 | 44.2 ± 8.8 47.0 ± 7.9 | 11.5 ± 2.4 11.9 ± 2.2 | 4.1 ± 1.9 4.2 ± 1.2 | 2.4 ± 0.8 2.6 ± 1.1 | 15.2 ± 2.4/83.8 ± 4.3 12.8 ± 3.1/86.2 ± 5.1 |
| B6 BMS + rat IgG | 14 28 | 1.2 ± 0.6 0.62 ± 0.2 | 68.6 ± 8.8 79.2 ± 8.4 | 6.4 ± 3.5 8.9 ± 3.6 | 30.1 ± 5.4 46.3 ± 6.1 | 16.2 ± 2.4 24.5 ± 3.3 | 31.7 ± 5.1 25.1 ± 4.5 | 1.4 ± 0.8/98.5 ± 1.1 6.3 ± 2.2/93.2 ± 4.5 |
| B6 BMS + anti-CD134L mAb | 14 28 | 1.0 ± 0.3 1.3 ± 0.2 | 32.1 ± 4.1 48.4 ± 5.3 | 29.8 ± 3.5 20.5 ± 4.6 | 14.1 ± 2.7 25.5 ± 4.3 | 4.6 ± 2.1 8.8 ± 3.1 | 21.5 ± 3.5 26.2 ± 3.8 | 1.5 ± 0.8/97.9 ± 2.1 1.8 ± 1.1/97.1 ± 3.1 |
| Mouse group . | Day . | No. of cells (×107) . | Cell population (% of total spleen cells) . | |||||
|---|---|---|---|---|---|---|---|---|
| CD3 . | CD19 . | CD4 . | CD134+CD4 . | CD8 . | H-2Kb+d+/H-2Kb+d− . | |||
| BDF1 (control) | 7.5 ± 1.6 | 52.1 ± 7.4 | 36.4 ± 8.1 | 35.6 ± 7.2 | 3.2 ± 0.8 | 17.8 ± 4.2 | 100/0 | |
| B6 TCD-BM | 14 28 | 6.5 ± 1.2 6.8 ± 1.4 | 14.9 ± 2.2 15.2 ± 3.5 | 44.2 ± 8.8 47.0 ± 7.9 | 11.5 ± 2.4 11.9 ± 2.2 | 4.1 ± 1.9 4.2 ± 1.2 | 2.4 ± 0.8 2.6 ± 1.1 | 15.2 ± 2.4/83.8 ± 4.3 12.8 ± 3.1/86.2 ± 5.1 |
| B6 BMS + rat IgG | 14 28 | 1.2 ± 0.6 0.62 ± 0.2 | 68.6 ± 8.8 79.2 ± 8.4 | 6.4 ± 3.5 8.9 ± 3.6 | 30.1 ± 5.4 46.3 ± 6.1 | 16.2 ± 2.4 24.5 ± 3.3 | 31.7 ± 5.1 25.1 ± 4.5 | 1.4 ± 0.8/98.5 ± 1.1 6.3 ± 2.2/93.2 ± 4.5 |
| B6 BMS + anti-CD134L mAb | 14 28 | 1.0 ± 0.3 1.3 ± 0.2 | 32.1 ± 4.1 48.4 ± 5.3 | 29.8 ± 3.5 20.5 ± 4.6 | 14.1 ± 2.7 25.5 ± 4.3 | 4.6 ± 2.1 8.8 ± 3.1 | 21.5 ± 3.5 26.2 ± 3.8 | 1.5 ± 0.8/97.9 ± 2.1 1.8 ± 1.1/97.1 ± 3.1 |
Phenotypic analysis was performed with use of fluorescence-activated cell sorter. Plus-minus values are the mean ± SD for 3 mice in each group. TCD-BM indicates T-cell-depleted bone marrow; BMS, bone marrow cells and spleen cells; and mAb, monoclonal antibody.