Secondary adoptive transfer of 205-immune BMT chimera lymphocytes into irradiated recipients does not induce GVHD
| Donor . | Vaccine Treated? . | # Dead/N . | # With Signs of Acute GVHD . | Final Weight Avg. ± SEM . |
|---|---|---|---|---|
| C3H.SW | No | 0/5 | 0/5 | 25 ± 0.9 |
| C3H.SW | Yes | 2/3* | 2/3 | 16 ± 3† |
| BMT chimera | No | 0/4 | 0/4 | 25 ± 0.9 |
| BMT chimera | Yes | 0/4 | 0/4 | 25 ± 1.5 |
| Donor . | Vaccine Treated? . | # Dead/N . | # With Signs of Acute GVHD . | Final Weight Avg. ± SEM . |
|---|---|---|---|---|
| C3H.SW | No | 0/5 | 0/5 | 25 ± 0.9 |
| C3H.SW | Yes | 2/3* | 2/3 | 16 ± 3† |
| BMT chimera | No | 0/4 | 0/4 | 25 ± 0.9 |
| BMT chimera | Yes | 0/4 | 0/4 | 25 ± 1.5 |
C3H.SW or long-term surviving C3H.SW → B6 BMT chimeras were either not immunized (nonimmune) or immunized (205-immune) with 5 × 106 irradiated 205IL-2/TK cells twice at a weekly interval. Ten days later, their splenocytes were adoptively transferred to C57BL/6 recipients that were given 850 cGy total body irradiation 1 day earlier. Survival was monitored until day 108. Final weights represent the weight of the surviving mice at day 108 or the weight at death for the 2 dead mice. Secondary transfer recipients were engrafted with C3H.SW T-cells as assessed by flow cytometry, which showed that the majority of Thy1.2+ cells in the spleen after sacrifice were CD5.1+.
P = .0431 compared to survival of recipients of nonimmune C3H.SW.
P < .01 compared to final weight of recipients of nonimmune C3H.SW.