Table 2. Polymerase chain reaction... | American Society of Hematology

Table 2.

Polymerase chain reaction primers for candidate genes in 5q31 deletion interval

Gene/EST	Primer Sequence (5′-3′)	Product Size (bp)	Annealing Temp (°C)	Mg²⁺ (mmol/L)
stSG30193^*	f: AGCCAGCCAGATCACAGAGT	125	56	1.5
	r: CACCCACCTCAACCAGAACT
stSG30427^†	f: TCTCCAAGAAACAGCCCG	150	53	2.5
	r: TTCACTCAGCACCTGGGAG
stSG30881^*	f: TTGCATCCTGGATATAATTCCC	157	56	1.5
	r: TCCCCTTTACTCAAATCTGGG
CDC25C^*	f: AACGGTCTTGCATAGCC	127	60	1.5
	r: CAGCCTTGAGTTGCATAGAG
SGC32445^*	f: AGTTTGGTTTATTGGCTCATCC	125	60	1.5
	r: TTCAGTCAGGGCCAGGAG
EGR1^*	f: GGAATCATGCCTTATGTAGTCAC	200	56	1.5
	r: GGACACATGACGTTTGCCTAGA
HSPA9^*	f: TCAGCAAGAGTGACATAGGAGAAGTGA	142	56	1.5
	r: CACAGCCTCATCAGGATTGACAGCTT
D5S1856^*	f: GGCTCAAGGGCTTTAGTCAA	299	57	3
	r: AGACTCCATCTTTCCAATAAAAATG
CTNNA1^*	f: CTTGGCCGCACCATTGCAGACCAT	464	56	1.5
	r: GGCCGGCCTGGGCAGACTTAGATG
stSG8723^†	f: CACCATCACCTATGCCCTCT	180	56	1.5
	r: ATAGGCAAAGCCACCCTTTT
WI-17966^*	f: ACAAAACTTGCCTGTACACTGC	128	54	1.5
	r: CAAGAGCCATTTTTCTTTTTGG
WI-15469^*	f: GCACATAATGCTTTATGTACCTGC	133	57	3
	r: GGTGATGATTTTAATGTGACATGC

Gene/EST	Primer Sequence (5′-3′)	Product Size (bp)	Annealing Temp (°C)	Mg²⁺ (mmol/L)
stSG30193^*	f: AGCCAGCCAGATCACAGAGT	125	56	1.5
	r: CACCCACCTCAACCAGAACT
stSG30427^†	f: TCTCCAAGAAACAGCCCG	150	53	2.5
	r: TTCACTCAGCACCTGGGAG
stSG30881^*	f: TTGCATCCTGGATATAATTCCC	157	56	1.5
	r: TCCCCTTTACTCAAATCTGGG
CDC25C^*	f: AACGGTCTTGCATAGCC	127	60	1.5
	r: CAGCCTTGAGTTGCATAGAG
SGC32445^*	f: AGTTTGGTTTATTGGCTCATCC	125	60	1.5
	r: TTCAGTCAGGGCCAGGAG
EGR1^*	f: GGAATCATGCCTTATGTAGTCAC	200	56	1.5
	r: GGACACATGACGTTTGCCTAGA
HSPA9^*	f: TCAGCAAGAGTGACATAGGAGAAGTGA	142	56	1.5
	r: CACAGCCTCATCAGGATTGACAGCTT
D5S1856^*	f: GGCTCAAGGGCTTTAGTCAA	299	57	3
	r: AGACTCCATCTTTCCAATAAAAATG
CTNNA1^*	f: CTTGGCCGCACCATTGCAGACCAT	464	56	1.5
	r: GGCCGGCCTGGGCAGACTTAGATG
stSG8723^†	f: CACCATCACCTATGCCCTCT	180	56	1.5
	r: ATAGGCAAAGCCACCCTTTT
WI-17966^*	f: ACAAAACTTGCCTGTACACTGC	128	54	1.5
	r: CAAGAGCCATTTTTCTTTTTGG
WI-15469^*	f: GCACATAATGCTTTATGTACCTGC	133	57	3
	r: GGTGATGATTTTAATGTGACATGC

RT-PCR performed using Boehringer Mannheim Titan One RT-PCR System.

RT-PCR performed using Sigma Enhanced Aviann RT-PCR Kit.