Checkerboard analysis of mast cell migration in response to NGF
| NGF in Lower Compartment (ng/mL) . | NGF in Upper Compartment (ng/mL) . | |||
|---|---|---|---|---|
| 0 . | 0.5 . | 5 . | 50 . | |
| 0 | 13 ± 3 | 23 ± 10 | 49 ± 11 | 72 ± 7 |
| 0.5 | 21 ± 5 | 23 ± 7 | 105 ± 30 | 67 ± 9 |
| 5 | 234 ± 41 | 83 ± 34 | 74 ± 9 | 98 ± 21 |
| 50 | 328 ± 73 | 177 ± 36 | 103 ± 21 | 65 ± 12 |
| NGF in Lower Compartment (ng/mL) . | NGF in Upper Compartment (ng/mL) . | |||
|---|---|---|---|---|
| 0 . | 0.5 . | 5 . | 50 . | |
| 0 | 13 ± 3 | 23 ± 10 | 49 ± 11 | 72 ± 7 |
| 0.5 | 21 ± 5 | 23 ± 7 | 105 ± 30 | 67 ± 9 |
| 5 | 234 ± 41 | 83 ± 34 | 74 ± 9 | 98 ± 21 |
| 50 | 328 ± 73 | 177 ± 36 | 103 ± 21 | 65 ± 12 |
Various concentrations of NGF were applied in both compartments, and migratory responses of PMCs were measured 3 hours later. Each value represents the mean ± SE of two separate experiments in triplicate.