HSCs from different donors demonstrate no donor effect in this SCM-based culture system
| BM Samples . | Culture Conditions . | Cell Number . | Frequency of CD34+thy-1+– Positive Wells . | Percentage of CD34+ thy-1+ Cells . |
|---|---|---|---|---|
| Donor #11 | Positive control | 335 000 ± 8000 | 43/48 | 9 ± 3 |
| Negative control | 88 000 ± 6000 | 1/48 | 1.6 | |
| 200% SCM-LIF | 358 000 ± 8000 | 46/48 | 18 ± 4 | |
| Donor #12 | Positive control | 346 000 ± 6000 | 42/48 | 8 ± 2 |
| Negative control | 80 000 ± 8000 | 2/48 | 1.8 ± 0.6 | |
| 200% SCM-LIF | 382 000 ± 9000 | 44/48 | 19 ± 4 | |
| Donor #13 | Positive control | 352 000 ± 10 000 | 43/48 | 10 ± 3 |
| Negative control | 90 000 ± 9000 | 1/48 | 1.2 | |
| 200% SCM-LIF | 378 000 ± 9000 | 43/48 | 19 ± 3 |
| BM Samples . | Culture Conditions . | Cell Number . | Frequency of CD34+thy-1+– Positive Wells . | Percentage of CD34+ thy-1+ Cells . |
|---|---|---|---|---|
| Donor #11 | Positive control | 335 000 ± 8000 | 43/48 | 9 ± 3 |
| Negative control | 88 000 ± 6000 | 1/48 | 1.6 | |
| 200% SCM-LIF | 358 000 ± 8000 | 46/48 | 18 ± 4 | |
| Donor #12 | Positive control | 346 000 ± 6000 | 42/48 | 8 ± 2 |
| Negative control | 80 000 ± 8000 | 2/48 | 1.8 ± 0.6 | |
| 200% SCM-LIF | 382 000 ± 9000 | 44/48 | 19 ± 4 | |
| Donor #13 | Positive control | 352 000 ± 10 000 | 43/48 | 10 ± 3 |
| Negative control | 90 000 ± 9000 | 1/48 | 1.2 | |
| 200% SCM-LIF | 378 000 ± 9000 | 43/48 | 19 ± 3 |
See Table 1 for details. These data were compiled from 3 independent experiments using different donor BM samples. For HSCs from each donor, two 24-well plates were initiated for each culture condition including positive control, negative control, and 200% SCM-LIF.