SCM-LIF maintains its activity to facilitate ex vivo expansion of freshly purified human fetal BM CD34+thy-1+ cells in the presence of SCM
| Culture Conditions . | Frequency of CD34+ thy-1+–Positive Wells . | Percentage of CD34+ thy-1+Cells . |
|---|---|---|
| Positive control | 100% (10/10) | 9 ± 3 |
| Negative control | 0% (0/10) | N/A |
| 200% SCM | 0% (0/10) | N/A |
| 400% SCM | 0% (0/10) | N/A |
| 200% SCM-LIF | 100% (10/10) | 17 ± 3 |
| 200% SCM-LIF + 200% SCM | 100% (10/10) | 17 ± 4 |
| 200% SCM-LIF + 400% SCM | 100% (10/10) | 18 ± 6 |
| Culture Conditions . | Frequency of CD34+ thy-1+–Positive Wells . | Percentage of CD34+ thy-1+Cells . |
|---|---|---|
| Positive control | 100% (10/10) | 9 ± 3 |
| Negative control | 0% (0/10) | N/A |
| 200% SCM | 0% (0/10) | N/A |
| 400% SCM | 0% (0/10) | N/A |
| 200% SCM-LIF | 100% (10/10) | 17 ± 3 |
| 200% SCM-LIF + 200% SCM | 100% (10/10) | 17 ± 4 |
| 200% SCM-LIF + 400% SCM | 100% (10/10) | 18 ± 6 |
See Table 1 for details.