Lymphoid reconstitution in γc-transduced mice
| Mouse Strain . | Peripheral Blood Percentages . | Spleen Cells (×106) . | Gut Cells % IEL . | ||
|---|---|---|---|---|---|
| Lymphocytes . | B Cells . | T Cells . | |||
| C57B1/6 (γc+) | 80 ± 12 | 39 ± 13 | 55 ± 12 | 60 ± 9 | 13 ± 4 |
| γc− | 8 ± 2 | 0.5 ± 0.1 | 10 ± 3.0 | 15 ± 11 | 0.3 ± 0.1 |
| RAG2/γc− | 0 ± 0 | 0 ± 0 | 0 ± 0 | 1.5 ± 0.2 | 0 ± 0 |
| γc-transduced | 48 ± 17 | 56 ± 33 | 40 ± 15 | 45 ± 17 | 4.3 ± 0.5 |
| γc+BM → RAG2/γc− | 79 ± 5.5 | 47 ± 3 | 47 ± 3 | 59 ± 15 | 15 ± 9 |
| Mouse Strain . | Peripheral Blood Percentages . | Spleen Cells (×106) . | Gut Cells % IEL . | ||
|---|---|---|---|---|---|
| Lymphocytes . | B Cells . | T Cells . | |||
| C57B1/6 (γc+) | 80 ± 12 | 39 ± 13 | 55 ± 12 | 60 ± 9 | 13 ± 4 |
| γc− | 8 ± 2 | 0.5 ± 0.1 | 10 ± 3.0 | 15 ± 11 | 0.3 ± 0.1 |
| RAG2/γc− | 0 ± 0 | 0 ± 0 | 0 ± 0 | 1.5 ± 0.2 | 0 ± 0 |
| γc-transduced | 48 ± 17 | 56 ± 33 | 40 ± 15 | 45 ± 17 | 4.3 ± 0.5 |
| γc+BM → RAG2/γc− | 79 ± 5.5 | 47 ± 3 | 47 ± 3 | 59 ± 15 | 15 ± 9 |
Results are the mean of 7 animals of each genotype. Peripheral blood cells were analyzed by staining with antibodies specific for B (anti-B220) and T (anti-CD3) cell antigens. Gut intraepithelial lymphocytes (IEL) were enumerated on histological sections and expressed per 100 epithelial cells. BM = bone marrow; RAG = recombinase activating gene.