Ligand stimulation of Notch affects differentiation, cell cycle, and apoptosis of the Lin− cells
| N = 5 . | Total Cells . | Live Cells . | Differentiation . | Cell Cycle . | Apoptosis . | ||
|---|---|---|---|---|---|---|---|
| Mac-1 . | Gr-1 . | G0/G1 . | S . | Sub-G1 . | |||
| GST | 426 (±19) | 284 (±31) | 84 (±2.5) | 76 (±6) | 86 (±3) | 7 (±1) | 46 (±6) |
| GSThDll1NDSL | 428 (±77) | 352 (±74) | 73 (±3.5) | 50 (±7) | 73 (±4) | 21 (±5) | 14 (±4) |
| P value | 0.9 | 0.04 | 0.007 | 0.002 | 0.01 | 0.01 | 0.005 |
| N = 5 . | Total Cells . | Live Cells . | Differentiation . | Cell Cycle . | Apoptosis . | ||
|---|---|---|---|---|---|---|---|
| Mac-1 . | Gr-1 . | G0/G1 . | S . | Sub-G1 . | |||
| GST | 426 (±19) | 284 (±31) | 84 (±2.5) | 76 (±6) | 86 (±3) | 7 (±1) | 46 (±6) |
| GSThDll1NDSL | 428 (±77) | 352 (±74) | 73 (±3.5) | 50 (±7) | 73 (±4) | 21 (±5) | 14 (±4) |
| P value | 0.9 | 0.04 | 0.007 | 0.002 | 0.01 | 0.01 | 0.005 |
Mouse Lin− bone marrow cells cultured for 7 days with GST or GST-hDll1NDSL were evaluated for their cellularity, expression of Mac-1 and Gr-1 antigens, cell-cycle kinetics, and apoptosis. Data are expressed as mean percentage of 5 independent experiments with SD in parentheses.