Percent of cell-type distribution before and after CD16 MACS of human granulocyte suspensions
| . | Before MACS . | CD16+ . | CD16− . | |||
|---|---|---|---|---|---|---|
| Control (n = 7) . | CML (n = 7) . | Control (n = 8) . | CML (n = 11) . | Control (n = 7) . | CML (n = 6) . | |
| Neutrophils | 92.1 ± 2.1 | 90.1 ± 4.3 | 99.1 ± 0.4 | 99.5 ± 0.3 | 10.7 ± 8.2 | 17.0 ± 5.2 |
| Eosinophils | 6.1 ± 1.6 | 7.0 ± 3.8 | 0 | 0 | 78.4 ± 9.7 | 63.2 ± 13.9 |
| Basophils | 0 | 0.7 ± 0.5 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0 | 2.7 ± 1.8 |
| Lymphocytes | 1.6 ± 0.8 | 1.1 ± 0.7 | 0.5 ± 0.3 | 0.2 ± 0.1 | 10.0 ± 6.8 | 4.0 ± 1.4 |
| Monocytes | 0.1 ± 0.2 | 0.4 ± 0.2 | 0 | 0.1 ± 0.1 | 0.7 ± 0.5 | 0.8 ± 0.5 |
| Metamyelocytes | 0 | 0.1 ± 0.2 | 0.1 ± 0.1 | 0 | 0.1 ± 0.2 | 2.0 ± 1.1 |
| Myelocytes | 0 | 0.4 ± 0.3 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0 | 7.9 ± 4.5 |
| Promyelocytes | 0 | 0 | 0 | 0 | 0 | 1.8 ± 1.1 |
| Blasts | 0 | 0 | 0 | 0.1 ± 0.1 | 0 | 0.7 ± 0.7 |
| . | Before MACS . | CD16+ . | CD16− . | |||
|---|---|---|---|---|---|---|
| Control (n = 7) . | CML (n = 7) . | Control (n = 8) . | CML (n = 11) . | Control (n = 7) . | CML (n = 6) . | |
| Neutrophils | 92.1 ± 2.1 | 90.1 ± 4.3 | 99.1 ± 0.4 | 99.5 ± 0.3 | 10.7 ± 8.2 | 17.0 ± 5.2 |
| Eosinophils | 6.1 ± 1.6 | 7.0 ± 3.8 | 0 | 0 | 78.4 ± 9.7 | 63.2 ± 13.9 |
| Basophils | 0 | 0.7 ± 0.5 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0 | 2.7 ± 1.8 |
| Lymphocytes | 1.6 ± 0.8 | 1.1 ± 0.7 | 0.5 ± 0.3 | 0.2 ± 0.1 | 10.0 ± 6.8 | 4.0 ± 1.4 |
| Monocytes | 0.1 ± 0.2 | 0.4 ± 0.2 | 0 | 0.1 ± 0.1 | 0.7 ± 0.5 | 0.8 ± 0.5 |
| Metamyelocytes | 0 | 0.1 ± 0.2 | 0.1 ± 0.1 | 0 | 0.1 ± 0.2 | 2.0 ± 1.1 |
| Myelocytes | 0 | 0.4 ± 0.3 | 0.1 ± 0.1 | 0.1 ± 0.1 | 0 | 7.9 ± 4.5 |
| Promyelocytes | 0 | 0 | 0 | 0 | 0 | 1.8 ± 1.1 |
| Blasts | 0 | 0 | 0 | 0.1 ± 0.1 | 0 | 0.7 ± 0.7 |
CD16+ neutrophils and eosinophil-enriched CD16− cell suspensions were prepared by MACS of the granulocyte fractions from CML patients and healthy controls. Duplicate cytocentrifugation slides were prepared from each cell fraction and stained with May-Grünwald-Giemsa. Thereafter, differential counting of a total of 200 cells per slide (ie, 400 cells per cell fraction from each individual) was performed. The numbers indicate percentage ± SEM of nucleated cells.