Table 1. The effect of TNF- on... | American Society of Hematology

Table 1.

The effect of TNF- on hematopoiesis is dependent on GM-CSF

Culture Additions	CFU-GM per 3 × 10⁵ Splenocytes + 2 × 10³14M1.4
Culture Additions	Uninfected 14M1.4	Infected 14M1.4
1. 60 μg/mL HIgG	24.2 ± 1.2	57.7 ± 3.8
2. 30 μg/mL α-GM-CSF + 30 μg/ml HIgG	10.3 ± 1.5^*	18.7 ± 1.8^*
3. 30 μg/mL HIgG + 30 μg/mL α-TNFα	27.0 ± 1.5	42.3 ± 2.0^*
4. 30 μg/mL α-GM-CSF + 30 μg/mL α-TNFα	10.3 ± 1.2^†	14.3 ± 3.2^†

Culture Additions	CFU-GM per 3 × 10⁵ Splenocytes + 2 × 10³14M1.4
Culture Additions	Uninfected 14M1.4	Infected 14M1.4
1. 60 μg/mL HIgG	24.2 ± 1.2	57.7 ± 3.8
2. 30 μg/mL α-GM-CSF + 30 μg/ml HIgG	10.3 ± 1.5^*	18.7 ± 1.8^*
3. 30 μg/mL HIgG + 30 μg/mL α-TNFα	27.0 ± 1.5	42.3 ± 2.0^*
4. 30 μg/mL α-GM-CSF + 30 μg/mL α-TNFα	10.3 ± 1.2^†	14.3 ± 3.2^†

14M1.4 cells were untreated or infected with amastigotes at a ratio of 25:1 (amastigotes/cells). 14M1.4 cells were preincubated with control HIgG, anti-GM-CSF mAb, or anti-TNF-α mAb for 30 minutes before addition of spleen cells in methylcellulose. After 7 days, mature CFU-GM colonies were counted. Data represents the mean ± SEM for triplicate wells.

P < 0.05 compared to HIgG control (row 1).

†

P < 0.05 compared to HIgG (row 1), but not significant compared to anti-GM-CSF (row 2).

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