Binding of FXa to activated platelets
| Platelets . | % Positive for FXa . | Mean Fluorescence . |
|---|---|---|
| Control | 0.8 ± 0.4 | 1.7 ± 0.3 |
| CVX | 19.5 ± 1.7 | 4.8 ± 1.1 |
| CVX + Thr (low-level FV) | 18.5 ± 1.0 | 4.4 ± 1.0 |
| CVX + Thr (COAT-FV) | 82.8 ± 5.9 | 20.1 ± 4.8 |
| Platelets . | % Positive for FXa . | Mean Fluorescence . |
|---|---|---|
| Control | 0.8 ± 0.4 | 1.7 ± 0.3 |
| CVX | 19.5 ± 1.7 | 4.8 ± 1.1 |
| CVX + Thr (low-level FV) | 18.5 ± 1.0 | 4.4 ± 1.0 |
| CVX + Thr (COAT-FV) | 82.8 ± 5.9 | 20.1 ± 4.8 |
Platelets were activated with 500 ng/mL convulxin (CVX) alone or in combination with 5 nmol/L thrombin (Thr) in the presence of 5 nmol/L FXa, 20 μg/mL FITC–anti-HFXa and 20 μg/mL biotin–anti-HFV237 as described in “Materials and methods.” Cells with surface-bound FV were identified as indicated in Figure 2. The percentage of cells positive for FXa as well as their mean FL1 fluorescence was then determined for each subpopulation of FV+ cells. Data are expressed as mean ± 1 SD; n = 3.