Effect of dual activation with thrombin and collagen on factor V expression on human platelets
| Collagen Type . | Low-Level Factor V . | COAT-FV . |
|---|---|---|
| I | 14.5 ± 5.6 | 36.4 ± 6.9 |
| III | 45.7 ± 13.8 | — |
| V | 19.4 ± 7.5 | 30.9 ± 9.6 |
| VI | 10.1 ± 3.9 | 61.6 ± 6.2 |
| Collagen Type . | Low-Level Factor V . | COAT-FV . |
|---|---|---|
| I | 14.5 ± 5.6 | 36.4 ± 6.9 |
| III | 45.7 ± 13.8 | — |
| V | 19.4 ± 7.5 | 30.9 ± 9.6 |
| VI | 10.1 ± 3.9 | 61.6 ± 6.2 |
To activate human platelets, 5 nmol/L thrombin and 20 μg/mL of collagen were used. Surface-bound FV was detected with monoclonal antibody HFV-237 (see “Materials and methods”), and the percentage of platelets expressing FV was then determined by flow cytometry. COAT-FV is defined in Figure 2 as events in region M2; low-level FV represents cells in region M1 minus those in region M2 of Figure 2. Data represent mean ± 1 SD, n = 4.