Table 1. Effect of survivin on caspase-3... | American Society of Hematology

Table 1.

Effect of survivin on caspase-3 activity

	Survivin₁₄₀ (nmole/L)	Survivin₁₂₁ (nmole/L)	Survivin₄₀ (nmole/L)	Caspase-3 Activity (ΔOD₄₀₅/10 min) (n = 3)
Caspase-3 (30 ng)	0	0	0	0.023 ± 0.002
	20	0	0	0.014 ± 0.003^*
	50	0	0	0^*
	0	20	0	0.016 ± 0.004^*
	0	50	0	0^*
	20	20	0	0^*
	0	0	50	0.021 ± 0.003
	20	20	50	0^*
Caspase-3 (0 ng)	50	0	0	0

	Survivin₁₄₀ (nmole/L)	Survivin₁₂₁ (nmole/L)	Survivin₄₀ (nmole/L)	Caspase-3 Activity (ΔOD₄₀₅/10 min) (n = 3)
Caspase-3 (30 ng)	0	0	0	0.023 ± 0.002
	20	0	0	0.014 ± 0.003^*
	50	0	0	0^*
	0	20	0	0.016 ± 0.004^*
	0	50	0	0^*
	20	20	0	0^*
	0	0	50	0.021 ± 0.003
	20	20	50	0^*
Caspase-3 (0 ng)	50	0	0	0

Purified recombinant myc/His₆-tagged survivins were assessed for their effect on caspase-3 activity using a chromogenic assay as detailed in “Materials and Methods.” Varying concentrations of survivins were tested. Results show a representative experiment done in triplicate, with associated standard errors. Survivin₁₄₀ and survivin₁₂₁ similarly suppressed caspase-3 activity in a dose-dependent manner, whereas survivin₄₀ had no effect. The asterisk denotes a significant difference in activity as compared with caspase-3 alone (first row) with P < .01.

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