Characteristics of changes in the 5′ noncoding regulatory region of the BCL-6 gene in non-Hodgkin lymphoma
| Sample . | Diagnosis . | Number of Evaluated BCL-6 Gene Clones . | Nucleotide Changes in Deletions, Insertions and Confirmed Substitution Mutations . | Number of Additional Single Unconfirmed Substitution Mutations . | Allelic Status . | Intraclonal Heterogeneity . |
|---|---|---|---|---|---|---|
| 1 | FCL | 8 | 75 (T → C), 105 (T → C), 123 (C → T), 190 (T → C),397 (G → C), 479 (G → C), 484 (T → C), 494 (T → G), 520 ▵T, 627 (C → G) | 5 | Heterozygous | + |
| 2 | FCL | 11 | 331 (G → A), 371 (C → T), 403 (G → A), 520 ▵T | 15 | Heterozygous | + |
| 3 | FCL | 8 | 397 (G → C),520 ▵T, 577 + C, 729 ΔC | 12 | Heterozygous | + |
| 4 | FCL | 7 | 397 (G → C), 437 (G → T) | 7 | Heterozygous | — |
| 5 | FCL | 9 | 123 (C → T), 173 (C → A), 292 (G → A), 611 (G → T), 690 (A → C) | 7 | Heterozygous | + |
| 6 | FCL | 5 | 397 (G → C) | 1 | Heterozygous | — |
| 7 | FCL | 8 | 186 (T → C) | 9 | Heterozygous | — |
| 8 | FCL | 8 | 186 (T → C), 586 (C → A), 671 (T → A) | 6 | Heterozygous | + |
| 9 | DLBCL | 9 | 520 ▵T, 639 (C → T), 411 ΔCTCGCGCT | 7 | Heterozygous | + |
| 10 | DLBCL | 8 | 4 | Homozygous | — | |
| 11 | DLBCL | 5 | 1 | Homozygous | — |
| Sample . | Diagnosis . | Number of Evaluated BCL-6 Gene Clones . | Nucleotide Changes in Deletions, Insertions and Confirmed Substitution Mutations . | Number of Additional Single Unconfirmed Substitution Mutations . | Allelic Status . | Intraclonal Heterogeneity . |
|---|---|---|---|---|---|---|
| 1 | FCL | 8 | 75 (T → C), 105 (T → C), 123 (C → T), 190 (T → C),397 (G → C), 479 (G → C), 484 (T → C), 494 (T → G), 520 ▵T, 627 (C → G) | 5 | Heterozygous | + |
| 2 | FCL | 11 | 331 (G → A), 371 (C → T), 403 (G → A), 520 ▵T | 15 | Heterozygous | + |
| 3 | FCL | 8 | 397 (G → C),520 ▵T, 577 + C, 729 ΔC | 12 | Heterozygous | + |
| 4 | FCL | 7 | 397 (G → C), 437 (G → T) | 7 | Heterozygous | — |
| 5 | FCL | 9 | 123 (C → T), 173 (C → A), 292 (G → A), 611 (G → T), 690 (A → C) | 7 | Heterozygous | + |
| 6 | FCL | 5 | 397 (G → C) | 1 | Heterozygous | — |
| 7 | FCL | 8 | 186 (T → C) | 9 | Heterozygous | — |
| 8 | FCL | 8 | 186 (T → C), 586 (C → A), 671 (T → A) | 6 | Heterozygous | + |
| 9 | DLBCL | 9 | 520 ▵T, 639 (C → T), 411 ΔCTCGCGCT | 7 | Heterozygous | + |
| 10 | DLBCL | 8 | 4 | Homozygous | — | |
| 11 | DLBCL | 5 | 1 | Homozygous | — |
Recurrent mutations are in bold; polymorphic changes, in italics.
FCL = follicular center lymphoma; DLBCL = diffuse large B-cell lymphoma.
Δ represent deletion.