vMIP-III is angiogenic in the chick embryo chorioallantoic membrane assay
| Sample* . | Dose (μg) . | Number of embryos . | Angiogenic response† . | Score‡ . | Percentage maximum score . | ||
|---|---|---|---|---|---|---|---|
| + . | +/− . | − . | |||||
| vMIP-III | 1.00 | 8 | 3 | 3 | 2 | 4.5 | 56.3 |
| 0.50 | 7 | 0 | 3 | 3 | 1.5 | 21.4 | |
| bFGF | 0.50 | 8 | 5 | 1 | 2 | 5.5 | 68.8 |
| 0.25 | 8 | 2 | 3 | 3 | 3.5 | 43.8 | |
| BSA | 2.00 | 8 | 1 | 1 | 6 | 1.5 | 18.8 |
| H2O | — | 10 | 0 | 0 | 10 | 0.0 | 0.0 |
| Sample* . | Dose (μg) . | Number of embryos . | Angiogenic response† . | Score‡ . | Percentage maximum score . | ||
|---|---|---|---|---|---|---|---|
| + . | +/− . | − . | |||||
| vMIP-III | 1.00 | 8 | 3 | 3 | 2 | 4.5 | 56.3 |
| 0.50 | 7 | 0 | 3 | 3 | 1.5 | 21.4 | |
| bFGF | 0.50 | 8 | 5 | 1 | 2 | 5.5 | 68.8 |
| 0.25 | 8 | 2 | 3 | 3 | 3.5 | 43.8 | |
| BSA | 2.00 | 8 | 1 | 1 | 6 | 1.5 | 18.8 |
| H2O | — | 10 | 0 | 0 | 10 | 0.0 | 0.0 |
Angiogenic induction was performed by methylcellulose disk containing recombinant vMIP-III, bFGF as a positive control. Disks containing BSA, vehicle alone (water) were used as negative controls.
Angiogenic responses were scored by 3 investigators: (+) unanimously positive; (+/−) split or unclear judgment; (−) unanimously negative.
Scores of 1, 0.5, and 0 were counted for (+), (+/−), and (−), respectively. The percentage maximum score is the division of the scores by the number of embryos.