As2O3-induced apoptosis in human myeloid leukemia cells
| . | % Apoptosis . | |||
|---|---|---|---|---|
| Control . | 0.5 μmol/L . | 2.0 μmol/L . | 10.0 μmol/L . | |
| HL-60/neo | 1.9 ± 1.5 | 7.9 ± 2.2 | 47.5 ± 3.6 | 86.1 ± 5.7 |
| HL-60/AR | 2.1 ± 0.5 | 8.5 ± 1.2 | 47.8 ± 2.1 | 78.5 ± 2.5 |
| HL-60/VCR | 2.3 ± 1.1 | 7.9 ± 1.2 | 50.3 ± 1.9 | 88.9 ± 2.3 |
| HL-60/Bcl-2 | 2.5 ± 0.6 | 4.5 ± 0.9 | 32.3 ± 1.1* | 85.5 ± 2.4 |
| HL-60/Bcl-xL | 2.9 ± 0.9 | 5.4 ± 1.3 | 35.6 ± 2.1* | 84.4 ± 2.7 |
| H-60/Bcr-Abl | 1.8 ± 2.3 | 3.6 ± 2.1 | 32.9 ± 2.9* | 72.2 ± 3.3 |
| K562 | 1.5 ± 1.0 | 3.5 ± 1.9 | 29.4 ± 2.2* | 70.9 ± 3.6 |
| . | % Apoptosis . | |||
|---|---|---|---|---|
| Control . | 0.5 μmol/L . | 2.0 μmol/L . | 10.0 μmol/L . | |
| HL-60/neo | 1.9 ± 1.5 | 7.9 ± 2.2 | 47.5 ± 3.6 | 86.1 ± 5.7 |
| HL-60/AR | 2.1 ± 0.5 | 8.5 ± 1.2 | 47.8 ± 2.1 | 78.5 ± 2.5 |
| HL-60/VCR | 2.3 ± 1.1 | 7.9 ± 1.2 | 50.3 ± 1.9 | 88.9 ± 2.3 |
| HL-60/Bcl-2 | 2.5 ± 0.6 | 4.5 ± 0.9 | 32.3 ± 1.1* | 85.5 ± 2.4 |
| HL-60/Bcl-xL | 2.9 ± 0.9 | 5.4 ± 1.3 | 35.6 ± 2.1* | 84.4 ± 2.7 |
| H-60/Bcr-Abl | 1.8 ± 2.3 | 3.6 ± 2.1 | 32.9 ± 2.9* | 72.2 ± 3.3 |
| K562 | 1.5 ± 1.0 | 3.5 ± 1.9 | 29.4 ± 2.2* | 70.9 ± 3.6 |
Various leukemia cell types were incubated with or without 0.5, 2.0, or 10.0 μmol/L As2O3 for 7 days. After this treatment, the percentage of total cells that were apoptotic was determined by annexin-V (excluding PI) staining and flow cytometry, as described in the text. Data represent the mean ± SD of 3 separate experiments.
Values are significantly different than those observed in HL-60/neo cells treated with 2.0 μmol/L As2O3 for 7 days (P < .05).