Phosphatase activities in sorted CD100+CD38+CD138− cells induced by activation of peripheral blood B lymphocytes
| Donor No. . | Immunoprecipitates . | ||
|---|---|---|---|
| CD100 . | CD45 . | CD148 . | |
| 1 | NS | 0.21 | 0.07 |
| 2 | NS | 0.28 | 0.54 |
| 3 | 0.11 | 0.66 | 0.36 |
| 4 | 0.11 | 1.38 | 0.76 |
| 5 | 0.20 | 0.40 | ND |
| 6 | 0.10 | 1.02 | ND |
| 7 | 0.17 | ND | ND |
| 8 | 0.07 | 1.14 | ND |
| 9 | NS | 0.40 | 0.55 |
| Donor No. . | Immunoprecipitates . | ||
|---|---|---|---|
| CD100 . | CD45 . | CD148 . | |
| 1 | NS | 0.21 | 0.07 |
| 2 | NS | 0.28 | 0.54 |
| 3 | 0.11 | 0.66 | 0.36 |
| 4 | 0.11 | 1.38 | 0.76 |
| 5 | 0.20 | 0.40 | ND |
| 6 | 0.10 | 1.02 | ND |
| 7 | 0.17 | ND | ND |
| 8 | 0.07 | 1.14 | ND |
| 9 | NS | 0.40 | 0.55 |
Nongranular and CD138− cells were isolated with the use of cell sorting from populations of activated B lymphocytes. Sorted cells corresponded to CD100+CD38+CD138− cells from small B lymphocyte and large B lymphocyte populations, as defined in Figure 1.
PTP activity was assayed in CD100, CD45, and CD148 immunoprecipitates (2 × 106 cells/100 μL of lysates) and is expressed as the absorbance at 405 nm. All experimental procedures are described in “Materials and Methods.”
NS, not significant value (absorbance < 0.05); ND, not determined.