The effect of AGN 193109 and ATRA on pre-CFU-S production from cultured lineage-negative, c-kit–positive, Sca-1–positive hematopoietic precursors
| Day of Culture . | ATRA . | AGN 193109 . | Number of Cells Injected in Primary Recipients . | Pre-CFU-S . |
|---|---|---|---|---|
| 0 | − | − | 500 | 8.33 ± 5.0 |
| 7 | − | − | 6.30 × 105 | 16.7 ± 1.37 |
| 7 | + | − | 2.75 × 105 | 3.33 ± 1.37 |
| 7 | − | + | 5.80 × 105 | 46.7 ± 4.90 |
| 14 | − | − | 1.88 × 106 | N/A |
| 14 | + | − | 3.32 × 106 | 4.50 ± 0.96 |
| 14 | − | + | 2.06 × 106 | N/A |
| Day of Culture . | ATRA . | AGN 193109 . | Number of Cells Injected in Primary Recipients . | Pre-CFU-S . |
|---|---|---|---|---|
| 0 | − | − | 500 | 8.33 ± 5.0 |
| 7 | − | − | 6.30 × 105 | 16.7 ± 1.37 |
| 7 | + | − | 2.75 × 105 | 3.33 ± 1.37 |
| 7 | − | + | 5.80 × 105 | 46.7 ± 4.90 |
| 14 | − | − | 1.88 × 106 | N/A |
| 14 | + | − | 3.32 × 106 | 4.50 ± 0.96 |
| 14 | − | + | 2.06 × 106 | N/A |
The total number of cells injected per primary recipient is given, being 500 lin− c-kit+ Sca-1+ precursor cells (day 0 of culture) or all cells that grew in liquid suspension culture from 500 or 1000 hematopoietic precursors at day 7 and 14, respectively. The culture medium was supplemented with 100 ng/mL SCF, IL-6, Flt-3L, and 10 ng/mL IL-11, without (−) or with (+) 1 μmol ATRA or 1 μmol AGN 193109. Pre-CFU-S are given as the total CFU-S produced per femur of primary recipients at day 12 after transplant in all secondary recipients (mean ± SEM, n = 4).
Only 1 mouse survived at this time point.
N/A = not available due to deaths of all mice before time of analysis.