The effect of AGN 193109 on CFU-S production from cultured lineage-negative, c-kit–positive, Sca-1–positive hematopoietic precursors
| Experiment 1 . | Experiment 2 . | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Day of Culture . | AGN 193109 . | Number of Cells Injected . | CFU-S D8 . | CFU-S D12 . | Day of Culture . | AGN 193109 . | Number of Cells Injected . | CFU-S D8 . | CFU-S D12 . |
| 0 | ND | ND | ND | ND | 0 | − | 500 | 4.25 ± 0.85 | 30.0 ± 10.0 |
| 7 | − | 6.90 × 105 | 14.0 ± 2.55 | 9.75 ± 1.25 | 7 | − | 4.80 × 105 | 21.0 ± 2.65 | 7.00 ± 0.00 |
| 7 | + | 6.80 × 105 | 28.0 ± 2.00 | 10.0 ± 4.0 | 7 | + | 4.90 × 105 | 32.3 ± 0.33 | 8.00 ± 0.58 |
| 14 | − | 2.26 × 106 | 1.00 ± 0.32 | 0* | 14 | − | 3.92 × 106 | 2.00 ± 1.15 | N/A |
| 14 | + | 2.32 × 106 | 0.40 ± 0.40 | 0* | 14 | + | 2.36 × 106 | 6.00 ± 1.15 | 1* |
| Experiment 1 . | Experiment 2 . | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Day of Culture . | AGN 193109 . | Number of Cells Injected . | CFU-S D8 . | CFU-S D12 . | Day of Culture . | AGN 193109 . | Number of Cells Injected . | CFU-S D8 . | CFU-S D12 . |
| 0 | ND | ND | ND | ND | 0 | − | 500 | 4.25 ± 0.85 | 30.0 ± 10.0 |
| 7 | − | 6.90 × 105 | 14.0 ± 2.55 | 9.75 ± 1.25 | 7 | − | 4.80 × 105 | 21.0 ± 2.65 | 7.00 ± 0.00 |
| 7 | + | 6.80 × 105 | 28.0 ± 2.00 | 10.0 ± 4.0 | 7 | + | 4.90 × 105 | 32.3 ± 0.33 | 8.00 ± 0.58 |
| 14 | − | 2.26 × 106 | 1.00 ± 0.32 | 0* | 14 | − | 3.92 × 106 | 2.00 ± 1.15 | N/A |
| 14 | + | 2.32 × 106 | 0.40 ± 0.40 | 0* | 14 | + | 2.36 × 106 | 6.00 ± 1.15 | 1* |
CFU-S are given as the total CFU-S counted per spleen at day 8 (D8) or day 12 (D12) posttransplant (mean ± sem; n = 5, experiment 1; n = 3, experiment 2). CFU-S after day 7 and 14 of culture is a measure from all cells that grew in liquid suspension culture from 500 or 1000 hematopoietic precursors, respectively. The total number of cells injected per mouse is given for each time point. The culture medium was supplemented with 100 ng/mL SCF, IL-6, Flt-3L and 10 ng/mL IL-11, without (−) or with (+) 1 μmol AGN 193109.
Only 1 mouse survived at this time point.
N/A = not available due to deaths of all mice before day 12. ND = not done.