Superoxide production in the cell-free system
| . | Membranes From Patient With Control Cytosol . | Cytosol From Patient With Control Membranes . | Membranes and Cytosol From Control . |
|---|---|---|---|
| JW (T341K) | 0.04 (±0.03) | 6.64 (±0.12) | 6.67 (±0.47) |
| CE (C369R) | 1.35 (±0.18) | 7.65 (±0.34) | 7.22 (±1.48) |
| MK (G408E) | 0.05 (±0.02) | 5.95 (±0.19) | 4.83 (±0.19) |
| DG (E568K) | 0.07 (±0.06) | 5.86 (±0.25) | 5.91 (±0.27) |
| . | Membranes From Patient With Control Cytosol . | Cytosol From Patient With Control Membranes . | Membranes and Cytosol From Control . |
|---|---|---|---|
| JW (T341K) | 0.04 (±0.03) | 6.64 (±0.12) | 6.67 (±0.47) |
| CE (C369R) | 1.35 (±0.18) | 7.65 (±0.34) | 7.22 (±1.48) |
| MK (G408E) | 0.05 (±0.02) | 5.95 (±0.19) | 4.83 (±0.19) |
| DG (E568K) | 0.07 (±0.06) | 5.86 (±0.25) | 5.91 (±0.27) |
Values are given in μmol /min (mean ± SD; n = 3). Neutrophil membranes (10 μg) and cytosol (200 μg) were incubated at 28°C in oxidase buffer, as previously described, in the presence of 60 μmol/L cytochrome c. After 2 minutes of incubation, SDS (100 μmol/L) and GTPγS (10 μmol/L) were added. After another 5 minutes, NADPH (250 μmol/L) was added, and the rate of cytochrome c reduction was determined by the change in absorbance at 550 nm.