Patients' characteristics and expression levels of surface IL-15R, IL-2Rβγ, and intracellular IL-15 in the malignant plasma cell fraction
| Patient No. . | Age (y) . | Paraprotein . | Bone Marrow Plasma Cells (%) . | IL-15Rα Expression (MFI) . | IL-2Rβ Expression (MFI) . | IL-2Rγ Expression (MFI) . | IL-15 Expression (MFI) . |
|---|---|---|---|---|---|---|---|
| #1 | 69 | IgAλ | 72 | 1.9 | 2.1 | 2.8 | 1.5 |
| #2 | 80 | IgGλ | 18 | 1.5 | 1.4 | 6.2 | 2.0 |
| #3 | 61 | IgGκ | 35 | 1.3 | 1.4 | 3.5 | 1.7 |
| #4 | 58 | IgAλ | 9 | 2.9 | 1.6 | 5.1 | negative |
| #5 | 64 | IgGλ | 56 | 2.0 | 1.3 | 2.9 | negative |
| #6 | 74 | IgGκ | 26 | 1.6 | 1.6 | 4.6 | negative |
| #7 | 63 | IgGκ | 35 | 3.4 | 1.7 | 3.9 | 3.8 |
| #8 | 54 | IgAλ | 10 | 3.3 | 1.5 | 6.5 | 3.0 |
| #9 | 64 | IgGλ | 82 | 1.3 | 1.4 | 4.6 | negative |
| #10 | 71 | IgAκ | 72 | 1.4 | 1.2 | 3.8 | negative |
| #11 | 69 | IgGκ | 8 | 3.2 | 1.2 | 2.8 | 1.4 |
| #12 | 76 | IgGλ | 19 | 2.8 | 1.4 | 2.2 | 1.5 |
| #13 | 43 | IgGκ | 12 | 3.3 | 1.9 | 2.1 | 2.2 |
| #14 | 73 | IgGκ | 20 | 1.5 | 1.3 | 2.5 | negative |
| Patient No. . | Age (y) . | Paraprotein . | Bone Marrow Plasma Cells (%) . | IL-15Rα Expression (MFI) . | IL-2Rβ Expression (MFI) . | IL-2Rγ Expression (MFI) . | IL-15 Expression (MFI) . |
|---|---|---|---|---|---|---|---|
| #1 | 69 | IgAλ | 72 | 1.9 | 2.1 | 2.8 | 1.5 |
| #2 | 80 | IgGλ | 18 | 1.5 | 1.4 | 6.2 | 2.0 |
| #3 | 61 | IgGκ | 35 | 1.3 | 1.4 | 3.5 | 1.7 |
| #4 | 58 | IgAλ | 9 | 2.9 | 1.6 | 5.1 | negative |
| #5 | 64 | IgGλ | 56 | 2.0 | 1.3 | 2.9 | negative |
| #6 | 74 | IgGκ | 26 | 1.6 | 1.6 | 4.6 | negative |
| #7 | 63 | IgGκ | 35 | 3.4 | 1.7 | 3.9 | 3.8 |
| #8 | 54 | IgAλ | 10 | 3.3 | 1.5 | 6.5 | 3.0 |
| #9 | 64 | IgGλ | 82 | 1.3 | 1.4 | 4.6 | negative |
| #10 | 71 | IgAκ | 72 | 1.4 | 1.2 | 3.8 | negative |
| #11 | 69 | IgGκ | 8 | 3.2 | 1.2 | 2.8 | 1.4 |
| #12 | 76 | IgGλ | 19 | 2.8 | 1.4 | 2.2 | 1.5 |
| #13 | 43 | IgGκ | 12 | 3.3 | 1.9 | 2.1 | 2.2 |
| #14 | 73 | IgGκ | 20 | 1.5 | 1.3 | 2.5 | negative |
Mean fluorescence intensities (MFI) of IL-15Rα, IL-2Rβγ, and IL-15 expression levels in malignant plasma cells (CD38high/CD45low) were determined by immunofluorescence staining and flow cytometric analysis and calculated as the ratio of MFI achieved with the specific antibody to isotype-matched control antibody. A ratio of 1.2 was considered positive.