Table 3.

AP1903 can support BFU-E colony development but not CFU-GM

BFU-E CFU-GM
No drugs or cytokines  0  16.4 
+AP1903  4  16  
+AP1903, SCF, IL-3  6.2  24  
SCF, IL-3  0  37.1  
Epo, SCF, IL-3  54  44 
BFU-E CFU-GM
No drugs or cytokines  0  16.4 
+AP1903  4  16  
+AP1903, SCF, IL-3  6.2  24  
SCF, IL-3  0  37.1  
Epo, SCF, IL-3  54  44 

Following transduction with the MFM vector, CD34-selected cord blood cells were plated in methyl-cellulose assays with cytokines or CID, as marked. The resulting colony numbers are displayed per 1000 cells. AP1903 alone was capable of supporting BFU-E development but not a CFU-GM development. The addition of SCF and IL-3 to the culture resulted in a synergistic effect on BFU-E development. It should be noted that 20% of colonies were transduced and therefore capable of responding to CID.

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