AP1903 can support BFU-E colony development but not CFU-GM
. | BFU-E . | CFU-GM . |
---|---|---|
No drugs or cytokines | 0 | 16.4 |
+AP1903 | 4 | 16 |
+AP1903, SCF, IL-3 | 6.2 | 24 |
SCF, IL-3 | 0 | 37.1 |
Epo, SCF, IL-3 | 54 | 44 |
. | BFU-E . | CFU-GM . |
---|---|---|
No drugs or cytokines | 0 | 16.4 |
+AP1903 | 4 | 16 |
+AP1903, SCF, IL-3 | 6.2 | 24 |
SCF, IL-3 | 0 | 37.1 |
Epo, SCF, IL-3 | 54 | 44 |
Following transduction with the MFM vector, CD34-selected cord blood cells were plated in methyl-cellulose assays with cytokines or CID, as marked. The resulting colony numbers are displayed per 1000 cells. AP1903 alone was capable of supporting BFU-E development but not a CFU-GM development. The addition of SCF and IL-3 to the culture resulted in a synergistic effect on BFU-E development. It should be noted that 20% of colonies were transduced and therefore capable of responding to CID.