Binding characteristics of solGMR produced by human leukemic cell lines
| Cell Line . | Kd, nmol . | Bmax, pmol . | # of Exp . |
|---|---|---|---|
| U937 | 1.7 ± 0.8 | 630 ± 390 | n = 6 |
| THP-1 | 3.6 ± 2.8 | 475 ± 390 | n = 5 |
| HL60 | 2.2 ± 0.7 | 550 ± 40 | n = 2 |
| Cell Line . | Kd, nmol . | Bmax, pmol . | # of Exp . |
|---|---|---|---|
| U937 | 1.7 ± 0.8 | 630 ± 390 | n = 6 |
| THP-1 | 3.6 ± 2.8 | 475 ± 390 | n = 5 |
| HL60 | 2.2 ± 0.7 | 550 ± 40 | n = 2 |
Supernatants conditioned by the cell lines U937, THP-1, and HL60 were subjected to 125I-GM-CSF saturation binding assays. Regression analysis of the data was used to determine the dissociation constant (Kd) and the concentration (Bmax) of soluble GM-CSF receptors. The number of independent experiments performed on supernatant from each cell line is shown (# of exp).