Table. Primers
| Bisulfite Conversion . | . | Sequencing . | PCR . |
|---|---|---|---|
| Coding strand | |||
| 5171F | 5′-TGGGTTTTTAGATTTAGTTATTTTGTGGAA-3′ | X | |
| 5196F | 5′-TGGAATTAAGTAATTTAGGTATTTTTGAGT-3′ | X | |
| 5547R | 5′-CTCTAACCAAAAATCAAAACTATTATCTAC-3′ | X | X |
| 5410FT | 5′-GTTTTAATTTAGGTGTTTTGTTTTTGTTTT-3′ | X | |
| 5446F | 5′-GGGTGGTTTTTATTTTTGGTGATTTTTTA-3′ | X | |
| 5893R | 5′-ACCCCAACCACCTCCTAACCAATA-3′ | X | X |
| 5686R | 5′-AACCTAAAAAAAAAAACGACTATCCA-3′ | X | |
| Noncoding strand | |||
| 5202F | 5′-TCAACAACCCAAACATCTCTAAATCTC-3′ | X | |
| 5264F | 5′-CAAAAACCCAACCTTTCCCAAATAACA-3′ | X | |
| 5566R | 5′-TGGTTTAGGGATTTTGCGGTTTTGGT-3′ | X | |
| 5546R | 5′-TTTGGTTGGGGGTTGGGGTTGTTATTTG-3′ | X | |
| 5410FB | 5′-ACCTCAACCCAAACATCCTACCCCTAC-3′ | X | |
| 5441F | 5′-AACCCCAAATAACCCCTACCCCTAAC-3′ | X | X |
| 5904R | 5′-GTCGGTTTTTGAATTTAGTTATTTTTTGGT-3′ | X | X |
| EMSAs | |||
| SP1 | 5′-ATTCGATCGGGGCGGGGCGAGC-3′ | ||
| HIF1 | 5′-GGCTGGGCCCTACGTGCTGTCTCACACAGCCTG-3′ | ||
| DR2 | 5′-CTGACCTCTCGACCTACCGGCCTAGGCCACA-3′ | ||
| P56 | 5′-GTCCTGCCCCTGCTCTGACCCCGGGTGGCCCCTACCCCTGGCGACCCCTCACGCACACAGC-3′ | ||
| LMPCR | |||
| BP4 | 5′-CTAGAGGAGAGGGCGGCTGTC-3′ | ||
| BP5 | 5′-GCGGTGTCGGAGCAGAGCGG-3′ | ||
| BP6 | 5′-TCGGAGCAGAGCGGGCGCGGTG-3′ | ||
| Bisulfite Conversion . | . | Sequencing . | PCR . |
|---|---|---|---|
| Coding strand | |||
| 5171F | 5′-TGGGTTTTTAGATTTAGTTATTTTGTGGAA-3′ | X | |
| 5196F | 5′-TGGAATTAAGTAATTTAGGTATTTTTGAGT-3′ | X | |
| 5547R | 5′-CTCTAACCAAAAATCAAAACTATTATCTAC-3′ | X | X |
| 5410FT | 5′-GTTTTAATTTAGGTGTTTTGTTTTTGTTTT-3′ | X | |
| 5446F | 5′-GGGTGGTTTTTATTTTTGGTGATTTTTTA-3′ | X | |
| 5893R | 5′-ACCCCAACCACCTCCTAACCAATA-3′ | X | X |
| 5686R | 5′-AACCTAAAAAAAAAAACGACTATCCA-3′ | X | |
| Noncoding strand | |||
| 5202F | 5′-TCAACAACCCAAACATCTCTAAATCTC-3′ | X | |
| 5264F | 5′-CAAAAACCCAACCTTTCCCAAATAACA-3′ | X | |
| 5566R | 5′-TGGTTTAGGGATTTTGCGGTTTTGGT-3′ | X | |
| 5546R | 5′-TTTGGTTGGGGGTTGGGGTTGTTATTTG-3′ | X | |
| 5410FB | 5′-ACCTCAACCCAAACATCCTACCCCTAC-3′ | X | |
| 5441F | 5′-AACCCCAAATAACCCCTACCCCTAAC-3′ | X | X |
| 5904R | 5′-GTCGGTTTTTGAATTTAGTTATTTTTTGGT-3′ | X | X |
| EMSAs | |||
| SP1 | 5′-ATTCGATCGGGGCGGGGCGAGC-3′ | ||
| HIF1 | 5′-GGCTGGGCCCTACGTGCTGTCTCACACAGCCTG-3′ | ||
| DR2 | 5′-CTGACCTCTCGACCTACCGGCCTAGGCCACA-3′ | ||
| P56 | 5′-GTCCTGCCCCTGCTCTGACCCCGGGTGGCCCCTACCCCTGGCGACCCCTCACGCACACAGC-3′ | ||
| LMPCR | |||
| BP4 | 5′-CTAGAGGAGAGGGCGGCTGTC-3′ | ||
| BP5 | 5′-GCGGTGTCGGAGCAGAGCGG-3′ | ||
| BP6 | 5′-TCGGAGCAGAGCGGGCGCGGTG-3′ | ||
Primers used for bisulfite conversion polymerase chain reaction and sequencing reactions have 100% converted sequences, except for primer 5409R, which contains 1 CpG near the 5′ end that was not converted. Primers are numbered according to their distances from the BamH1 site, 5788 bp upstream of the human erythropoietin translation start site. All oligonucleotides for electrophoretic mobility shift assay reactions were double stranded; only 1 strand is represented here.