Table 4. Immobilization on RN followed... | American Society of Hematology

Table 4.

Immobilization on RN followed by microinjection and detachment does not affect the ability of CD34⁺/CD38⁻ cells to produce colony forming cells

Method of Attachment^4-150	Method of Release	Microinjection	% Wells Producing Colony-Forming Cells^4-150	Colony Formation (% of Nonimmobilized Control)^4-151
Method of Attachment^4-150	Method of Release	Microinjection	% Wells Producing Colony-Forming Cells^4-150	Erythroid	Myeloid
—	—	−	74	100	100
			n = 42
RN	Peptide	−	68	79	86
			n = 40
RN	Peptide	+	77	132	100
			n = 64

Method of Attachment^4-150	Method of Release	Microinjection	% Wells Producing Colony-Forming Cells^4-150	Colony Formation (% of Nonimmobilized Control)^4-151
Method of Attachment^4-150	Method of Release	Microinjection	% Wells Producing Colony-Forming Cells^4-150	Erythroid	Myeloid
—	—	−	74	100	100
			n = 42
RN	Peptide	−	68	79	86
			n = 40
RN	Peptide	+	77	132	100
			n = 64

F4-150

Individual cells were transferred to wells of 96-well plates using the Quixell Transfer unit. To allow for the development and expansion of colony forming cells, transferred cells were cultured in Stem Cell Medium for 10 days before the addition of MethoCult GF media. After 14 days incubation in methylcellulose, the individual wells were evaluated for colony growth and phenotype. The total number of wells containing either erythroid or myeloid growth was determined for each condition.

F4-151

Colony formation is represented as the % of nonimmobilized control (number of wells giving rise to colony growth for treated cells ÷ number of wells giving rise to colony growth for suspension cells). Values represent combined results of 2 microinjection sessions in which the number of wells specified (n) were analyzed.

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