Table 3. Microinjection of fluorescent... | American Society of Hematology

Table 3.

Microinjection of fluorescent compounds into CD34⁺, CD34⁺/CD38⁻, and CD34⁺/CD38⁻/Thy-1^lo cells results in high cell viability

Cells	Matrix	Material Injected	Mode of Injection	Percent Viability^3-150,^3-151
Cells	Matrix	Material Injected	Mode of Injection	2-4 h	24 h	48 h
CD34⁺	RN	OG-dextran	Manual	67 ± 13	55 ± 8.5	52 ± 8.4
				(n = 63)	(n = 45)	(n = 45)
CD34⁺	RN	OG-dextran	Semiautomatic	n.d.	56 ± 5.2	54 ± 5.2
					(n = 6)	(n = 6)
CD34⁺	RN	Cy-3 IgG	Manual	70 ± 7.5	53 ± 6.2	44 ± 5.4
				(n = 3)	(n = 3)	(n = 3)
CD34⁺/CD38⁻	RN	OG-dextran	Manual	58 ± 23	41 ± 17	42 ± 14
				(n = 15)	(n = 9)	(n = 9)
CD34⁺/CD38⁻/Thy-1^lo	FN	FITC-dextran	Manual	28, 54	9, 52	3, 32

Cells	Matrix	Material Injected	Mode of Injection	Percent Viability^3-150,^3-151
Cells	Matrix	Material Injected	Mode of Injection	2-4 h	24 h	48 h
CD34⁺	RN	OG-dextran	Manual	67 ± 13	55 ± 8.5	52 ± 8.4
				(n = 63)	(n = 45)	(n = 45)
CD34⁺	RN	OG-dextran	Semiautomatic	n.d.	56 ± 5.2	54 ± 5.2
					(n = 6)	(n = 6)
CD34⁺	RN	Cy-3 IgG	Manual	70 ± 7.5	53 ± 6.2	44 ± 5.4
				(n = 3)	(n = 3)	(n = 3)
CD34⁺/CD38⁻	RN	OG-dextran	Manual	58 ± 23	41 ± 17	42 ± 14
				(n = 15)	(n = 9)	(n = 9)
CD34⁺/CD38⁻/Thy-1^lo	FN	FITC-dextran	Manual	28, 54	9, 52	3, 32

Values represent mean ± SD for number of separate plates (n), each containing 50 injected cells per plate.

Values shown in last row are, respectively, for 2 separate experiments of 32 and 50 injected cells per plate.