Primers Used for PCR Amplification and Sequencing of 3 Fibrinogen Polypeptide Genes
| Peptide and Exon(s) . | Forward (5′-3′) . | Reverse (5′-3′) . | Annealing Temperature (°C) . | PCR Product (bp) . |
|---|---|---|---|---|
| Aα | ||||
| I | GTC TAG GAG CCA GCC CCA CC | GTG TCA GGA CAT AGA GCA GG | 59 | 180 |
| II and III | GCC CTG TGT CTG CTC TCC TTA ATC | ACG AAA ACA AAA GCT CCC TC | 56 | 1,070 |
| IV and V(1) | TTT GCT GGC AAT TAC AGA CA | CCT GGG GCT TTC CGT CTC TG* | 56 | 1,144 |
| V(2) | AGA CCT GGT GGA AAT GAG ATT AC* | GTT AAG AAG GAA ATG CAA GGG | 53 | 1,206 |
| V(3) | TGG CAC TCT GAA TCT GGA AG*,† | CCC AGG GTG ATG AGA ACT GG*,† | ||
| Bβ | ||||
| I | GGA TGG TTT CTT GGA GC | GCT AAG CCA TCC TCA TCT | 50 | 335 |
| II and III | GAT TCA CTA TCA CCA ACC AGC CAG | CAT GCA TTC TCG TGG CAG TC | 50 | 1,088 |
| IV and V | CTG CTT GGT GAT AGC TCA GT | CTG GCC TTG TTT CCT GGC AT | 50 | 1,078 |
| VI and VII | GAA TGG ACA GGG GAT TCA GA | AAG TGC CCA GGA AGT GGT AG | 50 | 808 |
| VIII | GTG CAC ACG AGT GTA GCA GT | GAA CGC TTC TCC TTC CTT AC | 50 | 842 |
| γ | ||||
| I and II | GGA GCC TGA GAG GTG ACA GTG C | CCA GTT CAC ACA CAA AGG GAG AAA C | 50 | 321 |
| III and IV | TTT CTC TTT TAG TAT GTT GC | TCA ACA TAA TCA GGC ATA AT | 50 | 779 |
| V and VI | CTT ATT TTT GTC TTC TTA TT | TGT GCC TCA GTT TCC TTT TC | 50 | 784 |
| VII | ATT TTC TCC TTT TGC TCT TG | CCC AAG AAC CAA ACA GAC TC | 50 | 372 |
| VIII | AGA TCC CTG AGG AGG GTC AG | CTA AGA AAG GAA AAC ATA CC | 50 | 347 |
| IX and X | AGA CTT GCA GAG GTA AAA AG | GCT TTG CAA GTC CAT TGT CC | 55 | 880 |
| Peptide and Exon(s) . | Forward (5′-3′) . | Reverse (5′-3′) . | Annealing Temperature (°C) . | PCR Product (bp) . |
|---|---|---|---|---|
| Aα | ||||
| I | GTC TAG GAG CCA GCC CCA CC | GTG TCA GGA CAT AGA GCA GG | 59 | 180 |
| II and III | GCC CTG TGT CTG CTC TCC TTA ATC | ACG AAA ACA AAA GCT CCC TC | 56 | 1,070 |
| IV and V(1) | TTT GCT GGC AAT TAC AGA CA | CCT GGG GCT TTC CGT CTC TG* | 56 | 1,144 |
| V(2) | AGA CCT GGT GGA AAT GAG ATT AC* | GTT AAG AAG GAA ATG CAA GGG | 53 | 1,206 |
| V(3) | TGG CAC TCT GAA TCT GGA AG*,† | CCC AGG GTG ATG AGA ACT GG*,† | ||
| Bβ | ||||
| I | GGA TGG TTT CTT GGA GC | GCT AAG CCA TCC TCA TCT | 50 | 335 |
| II and III | GAT TCA CTA TCA CCA ACC AGC CAG | CAT GCA TTC TCG TGG CAG TC | 50 | 1,088 |
| IV and V | CTG CTT GGT GAT AGC TCA GT | CTG GCC TTG TTT CCT GGC AT | 50 | 1,078 |
| VI and VII | GAA TGG ACA GGG GAT TCA GA | AAG TGC CCA GGA AGT GGT AG | 50 | 808 |
| VIII | GTG CAC ACG AGT GTA GCA GT | GAA CGC TTC TCC TTC CTT AC | 50 | 842 |
| γ | ||||
| I and II | GGA GCC TGA GAG GTG ACA GTG C | CCA GTT CAC ACA CAA AGG GAG AAA C | 50 | 321 |
| III and IV | TTT CTC TTT TAG TAT GTT GC | TCA ACA TAA TCA GGC ATA AT | 50 | 779 |
| V and VI | CTT ATT TTT GTC TTC TTA TT | TGT GCC TCA GTT TCC TTT TC | 50 | 784 |
| VII | ATT TTC TCC TTT TGC TCT TG | CCC AAG AAC CAA ACA GAC TC | 50 | 372 |
| VIII | AGA TCC CTG AGG AGG GTC AG | CTA AGA AAG GAA AAC ATA CC | 50 | 347 |
| IX and X | AGA CTT GCA GAG GTA AAA AG | GCT TTG CAA GTC CAT TGT CC | 55 | 880 |