Table. In vitro attenuation of cytokine release in LPS-stimulated human whole blood by G-CSF
| . | LPS . | + G-CSF (ng/ml) . | ||||||
|---|---|---|---|---|---|---|---|---|
| 1 . | 3 . | 10 . | 100 . | 300 . | ||||
| TNF-α (ng/ml) | 9.4 ± 0.8 | 8.6 ± 1.1 | 6.8 ± 0.71-165 | 7.5 ± 0.71-165 | 6.1 ± 0.81-165 | 5.2 ± 0.71-165 | ||
| IL-12 (pg/ml) | 245 ± 54 | 237 ± 53 | 217 ± 52 | 201 ± 501-165 | 195 ± 471-165 | 174 ± 461-165 | ||
| IL-1β (ng/ml) | 39.1 ± 2.0 | 43.2 ± 3.1 | 37.0 ± 2.2 | 31.9 ± 1.91-165 | 34.3 ± 2.31-160 | 30.7 ± 2.21-165 | ||
| IFN-γ (ng/ml) | 26.8 ± 4.6 | 28.7 ± 5.8 | 20.0 ± 4.3* | 19.8 ± 3.6* | 18.1 ± 3.31-165 | 17.1 ± 3.41-165 | ||
| . | LPS . | + G-CSF (ng/ml) . | ||||||
|---|---|---|---|---|---|---|---|---|
| 1 . | 3 . | 10 . | 100 . | 300 . | ||||
| TNF-α (ng/ml) | 9.4 ± 0.8 | 8.6 ± 1.1 | 6.8 ± 0.71-165 | 7.5 ± 0.71-165 | 6.1 ± 0.81-165 | 5.2 ± 0.71-165 | ||
| IL-12 (pg/ml) | 245 ± 54 | 237 ± 53 | 217 ± 52 | 201 ± 501-165 | 195 ± 471-165 | 174 ± 461-165 | ||
| IL-1β (ng/ml) | 39.1 ± 2.0 | 43.2 ± 3.1 | 37.0 ± 2.2 | 31.9 ± 1.91-165 | 34.3 ± 2.31-160 | 30.7 ± 2.21-165 | ||
| IFN-γ (ng/ml) | 26.8 ± 4.6 | 28.7 ± 5.8 | 20.0 ± 4.3* | 19.8 ± 3.6* | 18.1 ± 3.31-165 | 17.1 ± 3.41-165 | ||
20% whole blood in RPMI 1640 was stimulated with 10 μg/ml LPS in the presence of different concentrations of G-CSF. After 24 h incubation at 37°C released cytokines were measured in the cell-free supernatants by ELISA. Data represent means ± SEM of 17 to 20 different donors.
p < .05,
p < .01,
p < .001 versus LPS control.