Proliferation of HUVEC and Other Primary Cells Exposed to Low-Dose Vinblastine
| Vinblastine Dose . | No. of Cells (×103) on Day 4* . | ||||
|---|---|---|---|---|---|
| HUVEC . | NIH 3T3 . | Namalwa . | LIK . | CEM . | |
| 0.25 pmol/L | 8.4 ± 3.2 | 68.2 ± 4.3 | 48.7 ± 4.6 | 37.4 ± 3.7 | 54.5 ± 5.1 |
| 1 pmol/L | 3.5 ± 1.1 | 58.5 ± 5.2 | 41.5 ± 5.8 | 44.9 ± 4.8 | 51.6 ± 4.2 |
| (33.2 ± 5.6) | (51.2 ± 4.7) | (42.3 ± 4.7) | (47.1 ± 6.7) | (59.8 ± 5.8) | |
| [4.1 ± 2.3] | [7.3 ± 2.8] | [11.2 ± 3.4] | [6.4 ± 1.8] | [8.3 ± 3.5] | |
| Vinblastine Dose . | No. of Cells (×103) on Day 4* . | ||||
|---|---|---|---|---|---|
| HUVEC . | NIH 3T3 . | Namalwa . | LIK . | CEM . | |
| 0.25 pmol/L | 8.4 ± 3.2 | 68.2 ± 4.3 | 48.7 ± 4.6 | 37.4 ± 3.7 | 54.5 ± 5.1 |
| 1 pmol/L | 3.5 ± 1.1 | 58.5 ± 5.2 | 41.5 ± 5.8 | 44.9 ± 4.8 | 51.6 ± 4.2 |
| (33.2 ± 5.6) | (51.2 ± 4.7) | (42.3 ± 4.7) | (47.1 ± 6.7) | (59.8 ± 5.8) | |
| [4.1 ± 2.3] | [7.3 ± 2.8] | [11.2 ± 3.4] | [6.4 ± 1.8] | [8.3 ± 3.5] | |
Results are expressed as the mean ± 1 SD in 1 representative experiment of 4 performed. Positive control values are in parentheses. Negative control values are in brackets.
The cells (2 × 103 per 0.32-cm2 well) were exposed to VBL on days 0 and 2 and counted on day 4, as described in the proliferation assay (Fig 1A).