Effect of MoAbs on vWF-Dependent CHO-GPIbβ/IX Cell Aggregation
| MoAb . | CHO-GPIbαβ/IX Aggregation (%) . | ||
|---|---|---|---|
| Buffer . | Ristocetin . | Heparin . | |
| None | 4.2 ± 0.8 | 51.0 ± 1.0 | 48 ± 4.9 |
| Control antibody | 3.1 ± 0.9 | 50.7 ± 0.9 | 47.8 ± 1.5 |
| 6D1 (anti-GPIbα) | 3.5 ± 1.2 | 5.3 ± 2.0* | 8.5 ± 2.5* |
| 328 (anti-vWF) | 5.8 ± 0.8 | 5.7 ± 3.7* | 10.6 ± 2.7† |
| 724 (anti-vWF) | 7.8 ± 0.8 | 50.3 ± 1.2 | 17.8 ± 1.6† |
| MoAb . | CHO-GPIbαβ/IX Aggregation (%) . | ||
|---|---|---|---|
| Buffer . | Ristocetin . | Heparin . | |
| None | 4.2 ± 0.8 | 51.0 ± 1.0 | 48 ± 4.9 |
| Control antibody | 3.1 ± 0.9 | 50.7 ± 0.9 | 47.8 ± 1.5 |
| 6D1 (anti-GPIbα) | 3.5 ± 1.2 | 5.3 ± 2.0* | 8.5 ± 2.5* |
| 328 (anti-vWF) | 5.8 ± 0.8 | 5.7 ± 3.7* | 10.6 ± 2.7† |
| 724 (anti-vWF) | 7.8 ± 0.8 | 50.3 ± 1.2 | 17.8 ± 1.6† |
CHO-GPIbαβ/IX cell aggregation was assessed at 6 cycles per second in the presence of 10 nmol/L vWF and either buffer, 1.4 mg/mL ristocetin or 100 μg/mL heparin. Cells were either preincubated with 20 μg/mL of control MoAb, 328, 724, or 6D1. The percentage of aggregated cells was calculated and the mean ± SEM was determined in 3 separate experiments. Statistical significance of differences between means was evaluated using the Student's t-test for paired samples by comparing aggregation in the presence of inhibitor versus the control corresponding sample in the absence of inhibitor.
P < .0025.
P < .05.