Table 2.

Superoxide Anion Production by 2 × 106Wild-Type and C/EBPɛ−/− Cells Collected From Either the Bone Marrow or Peritoneal Cavity (12 or 72 hours after intraperitoneal injection of 4% thioglycollate)

Granulocytes (%) Monocytes/ Macrophages (%)O2 Production (nmol/min) ±SDn
Bone marrow  
 Wild type  21  1  1.3 1.27  2  
 C/EBPε−/−  20  1  0* —  2  
Peritoneal phagocytes (12 h)  
 Wild type  70  30  5.95  0.08  2  
 C/EBPε−/− 56.5  43.5  1.09  0.01  2  
Peritoneal phagocytes (72 h)  
 Wild type  75  25  6.5  0.14  
 C/EBPε−/−  6  94  0.38  0.27  
Granulocytes (%) Monocytes/ Macrophages (%)O2 Production (nmol/min) ±SDn
Bone marrow  
 Wild type  21  1  1.3 1.27  2  
 C/EBPε−/−  20  1  0* —  2  
Peritoneal phagocytes (12 h)  
 Wild type  70  30  5.95  0.08  2  
 C/EBPε−/− 56.5  43.5  1.09  0.01  2  
Peritoneal phagocytes (72 h)  
 Wild type  75  25  6.5  0.14  
 C/EBPε−/−  6  94  0.38  0.27  

O2 production was measured by the continuous ferricytochrome c assay. Numbers represent mean and standard deviation of 2 separate experiments.

*

Undetectable.

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