Proliferative Response of Primary and Immortalized Monocytes to CD137
| . | Uncoated . | Fc . | CD137-Fc . |
|---|---|---|---|
| Primary monocytes | 0.4 ± 0.2 | 0.5 ± 0.2 | 5.1 ± 1.2 |
| HL-60 | 89.7 ± 2.7 | 95.5 ± 4.8 | 92.0 ± 12.8 |
| MonoMac | 224.0 ± 10.4 | 203.8 ± 28.9 | 225.1 ± 23.0 |
| . | Uncoated . | Fc . | CD137-Fc . |
|---|---|---|---|
| Primary monocytes | 0.4 ± 0.2 | 0.5 ± 0.2 | 5.1 ± 1.2 |
| HL-60 | 89.7 ± 2.7 | 95.5 ± 4.8 | 92.0 ± 12.8 |
| MonoMac | 224.0 ± 10.4 | 203.8 ± 28.9 | 225.1 ± 23.0 |
105 primary monocytes or Monomac or HL-60 cells were cultured on 96-well plates coated with Fc or CD137-Fc protein. Proliferation was determined at day 8 by a 24-hour pulse with 0.5 μCi3H-thymidine. Numbers represent means ± standard deviation of cpm × 1,000. This experiment was repeated 3 times with comparable results.