Effect of the Lack of Maternal GCs in Recolonization Capacity of Fetal Liver Thymic Precursors
| . | TcRαβhi . | % Mature Cells . | Cycling Cells . | |||
|---|---|---|---|---|---|---|
| %Total . | %DP . | CD4SP . | CD8SP . | %Total . | %TcRαβhi . | |
| Sham | 4.3 ± 0.7 | 3.3 ± 1.0 | 1.4 ± 0.3 | 0.6 ± 0.1 | 8.3 ± 1.3 | 15.1 ± 1.7 |
| Adx | 15.2 ± 2.1* | 10.6 ± 1.8† | 4.4 ± 0.8† | 3.0 ± 0.6† | 9.7 ± 2.4 | 14.2 ± 2.5 |
| . | TcRαβhi . | % Mature Cells . | Cycling Cells . | |||
|---|---|---|---|---|---|---|
| %Total . | %DP . | CD4SP . | CD8SP . | %Total . | %TcRαβhi . | |
| Sham | 4.3 ± 0.7 | 3.3 ± 1.0 | 1.4 ± 0.3 | 0.6 ± 0.1 | 8.3 ± 1.3 | 15.1 ± 1.7 |
| Adx | 15.2 ± 2.1* | 10.6 ± 1.8† | 4.4 ± 0.8† | 3.0 ± 0.6† | 9.7 ± 2.4 | 14.2 ± 2.5 |
Phenotype and cell cycle analysis of thymocytes generated in dGuo-treated murine thymic lobes after 12 days of in vitro recolonization with fetal liver cells from either 13-day old control Sham or Adx rat fetuses. In both cases, the percentage of rat cells yielded was ≥90%, without significant differences between lobes reconstituted with control or Adx fetal liver cells. No cross-reaction for mouse thymic molecules of used MoAbs against rat molecules was observed. Data represent the average values of 3 to 4 experiments ± SD.
P ≤ .01.
P ≤ .05.