Uptake of Exogenous Purified Rabbit Muscle TPI by Human Muscle and Brain Cell Lines
| Cell Line . | TPI Activity (U/μg protein) . | |
|---|---|---|
| Cultured in Serum-Free Medium . | Cultured in Serum-Free Medium + Rabbit Muscle TPI . | |
| Skeletal muscle | 26.7 ± 3 | 47.6 ± 6 |
| Brain (astrocytoma) | 10 ± 0.5 | 32 ± 6 |
| Cell Line . | TPI Activity (U/μg protein) . | |
|---|---|---|
| Cultured in Serum-Free Medium . | Cultured in Serum-Free Medium + Rabbit Muscle TPI . | |
| Skeletal muscle | 26.7 ± 3 | 47.6 ± 6 |
| Brain (astrocytoma) | 10 ± 0.5 | 32 ± 6 |
Cell lines were cultured in serum-free medium with or without (controls) rabbit muscle TPI. Rabbit muscle TPI (50 U in 1 mL medium) was added medium to muscle (1 × 104 cells) and brain (1 × 104 cells) cell lines and incubated as described in the Methods. Data represent means ± SD of 3 experiments in triplicate.