Expression of jak2a in Hematopoietic Mutants
| Mutant* . | Allele† . | Age of Onset‡ . | Stages Examined . | jak2a Expression1-153 . |
|---|---|---|---|---|
| cloche (clo) | m39 | 12 hpf | 13, 24, 48 hpf | Absent |
| spadetail (spt) | b104 | 12 hpf | 14, 19, 24 hpf | Absent |
| frascati (frs) | tm130d, tq223 | 2-4 dpf | 24, 36, 48 hpf, 3.5 dpf | Normal |
| chablis (cha) | tu245/tu242e | 2-3 dpf | 24, 36 hpf | Normal |
| retsina (ret) | tr217 | 3-4 dpf | 18, 24, 36, 48 hpf | Normal |
| cabernet (cab) | tl236 | 4 dpf | 24, 28 hpf | Normal |
| sauternes (sau) | ty121 | 2 dpf | 24, 26, 32, 36 hpf | Normal |
| wießherbst (weh) | th238 | 2 dpf | 24 hpf | Normal |
| chardonnay (cdy) | te216 | 2 dpf | 24, 36 hpf | Normal |
| chianti (cia) | tu25f | 3 dpf | 24, 36, 48 hpf | Normal |
| Mutant* . | Allele† . | Age of Onset‡ . | Stages Examined . | jak2a Expression1-153 . |
|---|---|---|---|---|
| cloche (clo) | m39 | 12 hpf | 13, 24, 48 hpf | Absent |
| spadetail (spt) | b104 | 12 hpf | 14, 19, 24 hpf | Absent |
| frascati (frs) | tm130d, tq223 | 2-4 dpf | 24, 36, 48 hpf, 3.5 dpf | Normal |
| chablis (cha) | tu245/tu242e | 2-3 dpf | 24, 36 hpf | Normal |
| retsina (ret) | tr217 | 3-4 dpf | 18, 24, 36, 48 hpf | Normal |
| cabernet (cab) | tl236 | 4 dpf | 24, 28 hpf | Normal |
| sauternes (sau) | ty121 | 2 dpf | 24, 26, 32, 36 hpf | Normal |
| wießherbst (weh) | th238 | 2 dpf | 24 hpf | Normal |
| chardonnay (cdy) | te216 | 2 dpf | 24, 36 hpf | Normal |
| chianti (cia) | tu25f | 3 dpf | 24, 36, 48 hpf | Normal |
The hematopoietic phenotype of the mutants examined here are described in Ransom et al,36 with the exception ofsau,38,clo,47,66,72,73 andspt.73 above).
The strongest allele was chosen for analysis of weh andret, whereas the weaker allele of sau was used. Thecha alleles tu245 and tu242e are believed to be clonal, and the frs alleles tm130d and tq223were tested both as homozygotes and trans-heterozygotes without observable differences.
The time in development when a clutch can be unambiguously scored as containing homozygous mutant embryos, taken from Ransom et al36 with the exception of clo and spt (see
Clutches of embryos from mutant incrosses were scored for disruption of jak2a expression by comparison with wild-type embryos of the same developmental stages. Absent indicates that there was nojak2a expression in any hematopoietic tissue at the stages examined. Normal indicates that there was no variation within a clutch or when compared with wild-type clutches.