CTL Lysis of HIV-Infected Primary CD4 T Cells Is Comparable to Lysis of Recombinant HIV-Vaccinia–Infected Autologous BLCL
| Clone T Cells . | Specificity . | Percent of Cells Expressing . | Percent Specific Cytotoxicity Versus . | ||
|---|---|---|---|---|---|
| CD28 . | CD57 . | Vaccinia- Infected BLCL . | HIV- Infected CD4 . | ||
| BR21 | RT | 61 | 13 | 61 | 41 |
| 352-env52 | env | 2 | 73 | 62 | 63 |
| 352-gag27 | gag | 32 | 74 | 31 | 50 |
| 352-nef26 | nef | ND | ND | 21 | 23 |
| Clone T Cells . | Specificity . | Percent of Cells Expressing . | Percent Specific Cytotoxicity Versus . | ||
|---|---|---|---|---|---|
| CD28 . | CD57 . | Vaccinia- Infected BLCL . | HIV- Infected CD4 . | ||
| BR21 | RT | 61 | 13 | 61 | 41 |
| 352-env52 | env | 2 | 73 | 62 | 63 |
| 352-gag27 | gag | 32 | 74 | 31 | 50 |
| 352-nef26 | nef | ND | ND | 21 | 23 |
CTL clones of diverse phenotypes recognizing 4 different HIV proteins were tested for specific cytotoxicity at an E:T ratio of 5:1 against either HIV-vaccinia infected autologous BLCL or immunomagnetically selected HIV-infected primary autologous CD4 T cells. Background lysis of the lacZ-vaccinia infected target or of the uninfected CD4 T cell blasts was less than 5% in all cases. The CTL clones were also analyzed by flow cytometry for expression of CD28 and CD57.
Abbreviation: ND, not done.