Characterizations of β2m-Induced Apoptosis and HLA Class I-Induced Apoptosis
| No.* . | MoAbs or Reagents . | Identical Epitope† . | Cells or Cell Lines . | Induction of Apoptosis . | Fas- Dependent . | Caspase(s)- Dependent . | Ceramide- Dependent . |
|---|---|---|---|---|---|---|---|
| (1) | Exogenous β2m | ND | K562, U937; CCRF-CEM | (+) | (−) | (+) | ND |
| (2) | Mouse MoAb90 Rat YTH862 | α1 domain | Activated peripheral B and T cells | (+) | (−) | (+) | (+) |
| (3) | Mouse CR11-351 Mouse RG1 | α2 domain | Jurkat E11‡ | (+) | (−) | (−) | ND |
| (4) | Mouse 5H7 | α3 domain | Peripheral T cells Jurkat BevD, JY and DBS-5211-153 14181-154 | (+) | (−) | (−) | (−) |
| (5) | Rabbit antihuman β2m | β2m (HLA class I) | Jurkat | (+) | (−) | (−) | ND |
| (6) | Mouse W6/32 | α2 + α3 domain | K562, CCRF-CEM | (−) | |||
| (7) | Mouse TU1491-155 | ND | K562, CCRF-CEM | (−) |
| No.* . | MoAbs or Reagents . | Identical Epitope† . | Cells or Cell Lines . | Induction of Apoptosis . | Fas- Dependent . | Caspase(s)- Dependent . | Ceramide- Dependent . |
|---|---|---|---|---|---|---|---|
| (1) | Exogenous β2m | ND | K562, U937; CCRF-CEM | (+) | (−) | (+) | ND |
| (2) | Mouse MoAb90 Rat YTH862 | α1 domain | Activated peripheral B and T cells | (+) | (−) | (+) | (+) |
| (3) | Mouse CR11-351 Mouse RG1 | α2 domain | Jurkat E11‡ | (+) | (−) | (−) | ND |
| (4) | Mouse 5H7 | α3 domain | Peripheral T cells Jurkat BevD, JY and DBS-5211-153 14181-154 | (+) | (−) | (−) | (−) |
| (5) | Rabbit antihuman β2m | β2m (HLA class I) | Jurkat | (+) | (−) | (−) | ND |
| (6) | Mouse W6/32 | α2 + α3 domain | K562, CCRF-CEM | (−) | |||
| (7) | Mouse TU1491-155 | ND | K562, CCRF-CEM | (−) |
Abbreviation: ND, not determined.
References: (1), (6), and (7), our study; (2) references 32, 33, 35, and 55; (3) reference 57; (4), references 5 and 39; and (5) references 58 and 59.
HLA class I heavy chain.
HLA-A2–expressing Jurkat cells.
EBV-transformed human lymphoblastoid cell lines.
B-cell line derived from a patient with Niemann-Pick disease.
Mouse monoclonal F(ab′)2 fragment.