Semiquantitative PCR Analysis of mCD24 Sequence Copy Number in Genomic DNA of Peripheral Blood Leukocytes From Animals Transplanted With Autologous CD34+ PBSC Transduced Ex Vivo With MFGS Retrovirus Vector Encoding mCD24
| 4694 . | 4649 . | 4657 . | J546 . | ||||
|---|---|---|---|---|---|---|---|
| Week . | PCR . | Week . | PCR . | Week . | PCR . | Week . | PCR . |
| 4 | 0.468 | 4 | 0.348 | 4 | 0.858 | 4 | 0.109 |
| 6 | 0.117 | 5 | 0.191 | 6 | 1.434 | 5 | 0.304 |
| 9 | 0.034 | 6.5 | 0.065 | 8 | 1.455 | 8 | 0.159 |
| 14 | 0.045 | 7.5 | 0.025 | 13 | 0.982 | 12 | 0.084 |
| 8.5 | 0.023 | 17 | 0.054 | 16 | 0 | ||
| 9.5 | 0.047 | 22 | 0.920 | 20 | 0 | ||
| 10.5 | 0.018 | ||||||
| 11.5 | 0.053 | ||||||
| 16 | 0.010 | ||||||
| 19 | 0.008 | ||||||
| 21 | 0.010 | ||||||
| 24 | 0.005 | ||||||
| 28 | 0.004 | ||||||
| 4694 . | 4649 . | 4657 . | J546 . | ||||
|---|---|---|---|---|---|---|---|
| Week . | PCR . | Week . | PCR . | Week . | PCR . | Week . | PCR . |
| 4 | 0.468 | 4 | 0.348 | 4 | 0.858 | 4 | 0.109 |
| 6 | 0.117 | 5 | 0.191 | 6 | 1.434 | 5 | 0.304 |
| 9 | 0.034 | 6.5 | 0.065 | 8 | 1.455 | 8 | 0.159 |
| 14 | 0.045 | 7.5 | 0.025 | 13 | 0.982 | 12 | 0.084 |
| 8.5 | 0.023 | 17 | 0.054 | 16 | 0 | ||
| 9.5 | 0.047 | 22 | 0.920 | 20 | 0 | ||
| 10.5 | 0.018 | ||||||
| 11.5 | 0.053 | ||||||
| 16 | 0.010 | ||||||
| 19 | 0.008 | ||||||
| 21 | 0.010 | ||||||
| 24 | 0.005 | ||||||
| 28 | 0.004 | ||||||
The copy number indicated is relative to the control standards as indicated in Fig 5 and in Materials and Methods. The level of accurate quantitative detection of signal as the assay is set up is a copy number of about 0.005 (marking of about 0.5% of cells).