Decreased CFU-C Frequencies After Fas Cross-Linking on IFNγ-Induced C57BL/6 Splenocytes
| . | CFU-C/106 Cells . |
|---|---|
| Control IgG | 47.2 ± 7.1 |
| Anti-Fas MoAb | 31.8 ± 2.1 |
| IFNγ | 42.0 ± 4.7 |
| Anti-Fas MoAb + IFNγ | 18.4 ± 2.97-150 |
| . | CFU-C/106 Cells . |
|---|---|
| Control IgG | 47.2 ± 7.1 |
| Anti-Fas MoAb | 31.8 ± 2.1 |
| IFNγ | 42.0 ± 4.7 |
| Anti-Fas MoAb + IFNγ | 18.4 ± 2.97-150 |
Spleen cells (107/mL) were distributed into 24-well culture plates and incubated for 24 hours in the presence of control IgG (5 μg/mL), anti-Fas MoAb (5 μg/mL), mrIFNγ (100 U/mL), or anti-Fas MoAb plus mrIFNγ. CFU-C were then recovered, enumerated, and assayed for colony growth in MethoCult M3434 medium. Data are means ± SEM from 5 separate experiments.
P < .01 between anti-Fas + IFNγ and control IgG.