Distribution of CRU Between the Undivided and Postmitotic Fractions of Cells Obtained From Five-Day Cultures of CD34+ Fetal Liver and Cord Blood Cells That Had Not Divided During This Period (Undivided Cells) and That Had (Postmitotic)
| Origin of CD34+ Cells Cultured . | Experiment No. . | Undivided Cells . | Postmitotic Cells . | |||||
|---|---|---|---|---|---|---|---|---|
| Cells Injected per Mouse (×103) . | B+ and M+ . | B+ Only . | Cells Injected per Mouse (×103) . | B+ and M+ . | Level of Engraftment (%) . | B+ Only . | ||
| Fetal liver | 2 | 0.7 | 0/3 | 0/3 | 250 | 0/8 | — | 5/8 |
| Fetal liver | 3 | 6.3 | 0/5 | 0/5 | 670 | 2/7 | 0.1, 8 | 5/7 |
| Cord blood | 2 | 3.5 | 0/4 | 0/4 | 370 | 1/4 | 5 | 4/4 |
| 1,400 | 1/1 | 83 | 1/1 | |||||
| Cord blood | 3 | 0.4 | 0/4 | 1/4 | 1,300 | 3/5 | 5, 5, 13 | 4/5 |
| Origin of CD34+ Cells Cultured . | Experiment No. . | Undivided Cells . | Postmitotic Cells . | |||||
|---|---|---|---|---|---|---|---|---|
| Cells Injected per Mouse (×103) . | B+ and M+ . | B+ Only . | Cells Injected per Mouse (×103) . | B+ and M+ . | Level of Engraftment (%) . | B+ Only . | ||
| Fetal liver | 2 | 0.7 | 0/3 | 0/3 | 250 | 0/8 | — | 5/8 |
| Fetal liver | 3 | 6.3 | 0/5 | 0/5 | 670 | 2/7 | 0.1, 8 | 5/7 |
| Cord blood | 2 | 3.5 | 0/4 | 0/4 | 370 | 1/4 | 5 | 4/4 |
| 1,400 | 1/1 | 83 | 1/1 | |||||
| Cord blood | 3 | 0.4 | 0/4 | 1/4 | 1,300 | 3/5 | 5, 5, 13 | 4/5 |
Level of engraftment (%) refers to the percent of human CD45+ cells detected in the marrow of mice in which both human B-lineage (CD19/20+) and myeloid (CD15/66b+) cells were detected. The (average) relative proportion of the total culture in each of the 2 fractions compared can be readily derived from the data shown in Fig 3. In these experiments, 83% to 90% of all the cells recovered in each experiment were used to assess the distribution of CRU between the 2 fractions being compared.