Immunosuppressive Effect of CTLA4Ig in MLC
| . | Protein (μg/mL) . | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 . | 0.15 . | 0.31 . | 0.625 . | 1.25 . | 2.50 . | 5.00 . | 10.00 . | |
| CTLA4-Ig (cpm) | ||||||||
| Experiment 1 | 25,346 | 10,254 | 2,536 | 1,181 | 1,333 | 1,193 | 1,126 | 700 |
| Experiment 2 | 8,091 | 5,190 | 3,331 | 1,182 | 473 | 328 | 179 | 334 |
| L-6 (cpm) | 14,607 | 20,956 | 19,360 | 14,265 | 19,568 | 20,332 | 13,393 | 13,326 |
| . | Protein (μg/mL) . | |||||||
|---|---|---|---|---|---|---|---|---|
| 0 . | 0.15 . | 0.31 . | 0.625 . | 1.25 . | 2.50 . | 5.00 . | 10.00 . | |
| CTLA4-Ig (cpm) | ||||||||
| Experiment 1 | 25,346 | 10,254 | 2,536 | 1,181 | 1,333 | 1,193 | 1,126 | 700 |
| Experiment 2 | 8,091 | 5,190 | 3,331 | 1,182 | 473 | 328 | 179 | 334 |
| L-6 (cpm) | 14,607 | 20,956 | 19,360 | 14,265 | 19,568 | 20,332 | 13,393 | 13,326 |
A total of 105 responder cells and 105irradiated (2,000 cGy) stimulator cells obtained from DLA-nonidentical unrelated dogs were cultured per well either in the absence or the presence of increasing concentrations of CTLA4Ig or the control peptide L-6. 3H-thymidine incorporation was determined after 6 days of culture. Responses are presented as the mean cpm of triplicate experiments. CTLA4Ig was tested in 2 separate experiments.