Summary of Binding and Functional Activities of CCR5 Ligands
| CC-Chemokines . | pIC50 (−Log M) ± s.e.m. . | pEC50 (−Log M) ± s.e.m. . |
|---|---|---|
| RANTES | 9.74 ± 0.19 | 8.87 ± 0.37 |
| MIP-1α | 9.05 ± 0.24 | 8.49 ± 0.47 |
| MIP-1β | 9.30 ± 0.24 | 8.47 ± 0.40 |
| MCP-2 | 9.40 ± 0.35 | 8.44 ± 0.32 |
| MCP-3 | 8.59 ± 0.11 | <6.3 |
| MCP-4 | 8.03 ± 0.28 | 7.04 ± 0.27 |
| Eotaxin | 7.72 ± 0.10 | <6.3 |
| MCP-1 | 7.34 ± 0.25 | <6.3 |
| CC-Chemokines . | pIC50 (−Log M) ± s.e.m. . | pEC50 (−Log M) ± s.e.m. . |
|---|---|---|
| RANTES | 9.74 ± 0.19 | 8.87 ± 0.37 |
| MIP-1α | 9.05 ± 0.24 | 8.49 ± 0.47 |
| MIP-1β | 9.30 ± 0.24 | 8.47 ± 0.40 |
| MCP-2 | 9.40 ± 0.35 | 8.44 ± 0.32 |
| MCP-3 | 8.59 ± 0.11 | <6.3 |
| MCP-4 | 8.03 ± 0.28 | 7.04 ± 0.27 |
| Eotaxin | 7.72 ± 0.10 | <6.3 |
| MCP-1 | 7.34 ± 0.25 | <6.3 |
The pIC50 (−log M) values were obtained from competition binding experiments, using [125I]-MIP-1β as a tracer (as displayed in Fig 1). The pEC50 (−log M) values were obtained from functional dose-response curves using the aequorin assay (as displayed in Fig 2A). Values represent the mean and s.e.m. of at least two (pIC50) or three (pEC50) independent determinations. One representative experiment is displayed in Fig 1.