The Basal Activities of Antioxidant Enzymes and As2O3-Induced Apoptosis in Different Human Leukemia Cell Lines
| Cell Lines . | GSTπ . | GPx . | Catalase . | Apoptosis . |
|---|---|---|---|---|
| NB4 | 94.0 ± 8.37 | 28.3 ± 5.4 | 25.8 ± 9.3 | 67.5 ± 5.8 |
| HL-60 | 138.6 ± 1.9 | 55.5 ± 10.2 | 198.3 ± 10.6 | 5.8 ± 2.1 |
| U937 | 212.1 ± 15.3 | 67.6 ± 1.5 | 170.5 ± 9.9 | 5.6 ± 3.4 |
| KG1 | 176.2 ± 5.1 | 82.8 ± 10.6 | 108.9 ± 7.3 | 17.5 ± 4.2 |
| K562 | 232.3 ± 6.9 | 12.3 ± 8.6 | 85.5 ± 8.9 | 6.7 ± 3.1 |
| Cell Lines . | GSTπ . | GPx . | Catalase . | Apoptosis . |
|---|---|---|---|---|
| NB4 | 94.0 ± 8.37 | 28.3 ± 5.4 | 25.8 ± 9.3 | 67.5 ± 5.8 |
| HL-60 | 138.6 ± 1.9 | 55.5 ± 10.2 | 198.3 ± 10.6 | 5.8 ± 2.1 |
| U937 | 212.1 ± 15.3 | 67.6 ± 1.5 | 170.5 ± 9.9 | 5.6 ± 3.4 |
| KG1 | 176.2 ± 5.1 | 82.8 ± 10.6 | 108.9 ± 7.3 | 17.5 ± 4.2 |
| K562 | 232.3 ± 6.9 | 12.3 ± 8.6 | 85.5 ± 8.9 | 6.7 ± 3.1 |
The units were nanomoles per milligram of protein per minute for glutathione-S-transferaseπ (GSTπ); milliunits per minute per milligram of protein for glutathione peroxidase (GPx); micromoles of H2O2 decomposed per milligram of protein per minute for catalase. Apoptosis (percentage of apoptotic cells) was quantitated by fluorescence microscopy in cultures treated for 2 days with 2 μmol/L As2O3. All data shown represent the mean ± SE of 3 independent experiments.