Concentrations of TPO Protein in the Supernatant of BM Stromal Cells Stimulated With TGF-β1 or Platelet Lysate
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Bone marrow stromal cells were suspended in 96-well plates at a concentration of 5 × 104/250 μL per well. After 24 hours of culture, the cells were refed with or without active TGF-β1 or platelet lysate. The concentration of TGF-β1 in platelet lysate was diluted to 2.0 ng/mL with α-MEM. The supernatants were removed after 24 and 48 hours of incubation and then assessed for ELISA. The platelet lysates were preincubated with 100 mg/mL of a polyclonal neutralizing anti–TGF-β1 antibody or an irrelevant mouse IgG1 at 37°C for 2 hours. Numbers represent TPO concentrations in picograms per milliliter.
Abbreviation: ND, not detectable.
P < .05.