Cell Surface Markers on Donor Macrophages
| Marker . | Peritoneal* . | Progenitor† . | ||
|---|---|---|---|---|
| % Positive . | Intensity‡ . | % Positive . | Intensity‡ . | |
| Mac-1 | ≥90 | +-+++ | ≥90 | +-+++ |
| Mac-3 | ≥90 | +++ | ≥90 | +-++ |
| F4/80 | ≥90 | +-+++ | 0 | 0 |
| MME | 5-10 | +++ | 0 | 0 |
| Marker . | Peritoneal* . | Progenitor† . | ||
|---|---|---|---|---|
| % Positive . | Intensity‡ . | % Positive . | Intensity‡ . | |
| Mac-1 | ≥90 | +-+++ | ≥90 | +-+++ |
| Mac-3 | ≥90 | +++ | ≥90 | +-++ |
| F4/80 | ≥90 | +-+++ | 0 | 0 |
| MME | 5-10 | +++ | 0 | 0 |
Peritoneal macrophages refer to cells obtained from the peritoneum of mice 5 days after thioglycollate stimulation.
Progenitor macrophages refer to mononuclear cells derived from bone marrow exposed to IL-3 and M-CSF in vitro.
0, no staining; +, minimal; ++, moderate; +++, intense staining above background.