FAD Binding of WT and ▵488-497 gp91 phox Flavocytochromeb558
| Source of Flavocytochrome b558 . | Kd (FAD) . |
|---|---|
| WT gp91 phoxPLB-985 | 66.9 ± 1.7 nmol/L |
| Δ488-497 gp91 phox PLB-985 | 65.3 ± 2.2 nmol/L |
| X-CGD PLB-9853-150 | No binding |
| Source of Flavocytochrome b558 . | Kd (FAD) . |
|---|---|
| WT gp91 phoxPLB-985 | 66.9 ± 1.7 nmol/L |
| Δ488-497 gp91 phox PLB-985 | 65.3 ± 2.2 nmol/L |
| X-CGD PLB-9853-150 | No binding |
An equivalent volume of the X-CGD PLB-985 cell fractions eluted from the heparin column that corresponding to the peak fractions of the Δ488-497 gp91 phox and WT gp91 phox flavocytochromeb558 was used. The concentration of FAD was 51.3 nmol/L in the buffer chamber and 53 nmol/L in the sample chamber after dialysis.