Superoxide Production in the Cell-Free Oxidase Assay Using Cellular Membranes and Cytosol Isolated From Transgenic PLB-985 Cells
| Source of Membranes . | Source of Cytosol . | |
|---|---|---|
| Δ488-497 gp91 phox PLB-985 . | WT gp91 phox PLB-985 . | |
| WT gp91 phox PLB-985 | 183.4 ± 16.8* | 165.6 ± 20.3 |
| Δ488-497 gp91 phoxPLB-985 | 3.8 ± 1.5 | 4.3 ± 2.1 |
| Source of Membranes . | Source of Cytosol . | |
|---|---|---|
| Δ488-497 gp91 phox PLB-985 . | WT gp91 phox PLB-985 . | |
| WT gp91 phox PLB-985 | 183.4 ± 16.8* | 165.6 ± 20.3 |
| Δ488-497 gp91 phoxPLB-985 | 3.8 ± 1.5 | 4.3 ± 2.1 |
After 5 days of induction with DMF (0.5%), the cytosolic and membrane fractions were separated from the indicated cells and used in the cell-free oxidase reconstitution assay. Ten micrograms of membranes and 20 μg of cytosol were used in the assay. The data represent the mean ± SD of 3 separate experiments.
Units: nmol/min/mg membrane proteins.