PCR Primers and the TPO Isoforms Amplified
| 5′ Primers . | 3′ Primers . | Type of Isoform . | Size of PCR Product (bp) . |
|---|---|---|---|
| S3: 5′-TCCTAACTGCAAGGCTAACG-3′ | A3: 5′-AGGGACCTGGAGGTTTGGTT-3′ | ||
| S7: 5′-TGCAGAGCCTCCTTGGAACC-3′ | A2: 5′-CTGAATCCCTGCTGCCACTT-3′ | TPO-1 | 304 |
| TPO-2 | 292 | ||
| TPO-3 | 188 | ||
| TPO-4 | 107 | ||
| TPO-5 | 176 | ||
| TPO-6 | 223 | ||
| S2: 5′-TTCACCCTTTGCCTACACCT-3′ | A4: 5′-GCTGTGGTCCTGCCCTGGGT-3′ | TPO-2 | 253 |
| 5′ Primers . | 3′ Primers . | Type of Isoform . | Size of PCR Product (bp) . |
|---|---|---|---|
| S3: 5′-TCCTAACTGCAAGGCTAACG-3′ | A3: 5′-AGGGACCTGGAGGTTTGGTT-3′ | ||
| S7: 5′-TGCAGAGCCTCCTTGGAACC-3′ | A2: 5′-CTGAATCCCTGCTGCCACTT-3′ | TPO-1 | 304 |
| TPO-2 | 292 | ||
| TPO-3 | 188 | ||
| TPO-4 | 107 | ||
| TPO-5 | 176 | ||
| TPO-6 | 223 | ||
| S2: 5′-TTCACCCTTTGCCTACACCT-3′ | A4: 5′-GCTGTGGTCCTGCCCTGGGT-3′ | TPO-2 | 253 |
S3 and A3 primers were used for the first PCR of the cDNA pool. Combinations of S7-A2 and S2-A4 primers were used for the second PCR (nested-PCR) of the first PCR products.